PUBLICATION
Neu1 deficiency increases the susceptibility of zebrafish to Edwardsiella piscicida infection via lysosomal dysfunction
- Authors
- Sahashi, D., Kubo, Y., Ishi, M., Ikeda, A., Yamasaki, C., Komatsu, M., Shiozaki, K.
- ID
- ZDB-PUB-220622-30
- Date
- 2022
- Source
- Gene 836: 146667 (Journal)
- Registered Authors
- Shiozaki, Kazuhiro
- Keywords
- lysosome, oligosaccharide, sialic acid, sialidase, zebrafish
- MeSH Terms
-
- Animals
- Edwardsiella
- Lysosomes
- Mucolipidoses
- Neuraminidase*/genetics
- Neuraminidase*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 35714800 Full text @ Gene
Citation
Sahashi, D., Kubo, Y., Ishi, M., Ikeda, A., Yamasaki, C., Komatsu, M., Shiozaki, K. (2022) Neu1 deficiency increases the susceptibility of zebrafish to Edwardsiella piscicida infection via lysosomal dysfunction. Gene. 836:146667.
Abstract
Neu1 is a lysosomal glycosidase that catalyzes the removal of sialic acids from glycoconjugates. Although Neu1 sialidase is highly conserved among vertebrates, the role of fish Neu1 is not fully understood because of its unique aquatic living situation. Compared to land animals, fish have a higher chance of bacterial infection, and to understand the role of fish Neu1, the susceptibility of Neu1 knockout zebrafish (Neu1-KO) was evaluated using Edwardsiella piscicida, a fish pathogen. Neu1-KO larvae showed high susceptibility to E. piscicida, despite the activation of macrophages, and presented increased lysosomal signals induced by the accumulation of Sia α2-3 linked oligosaccharides. The accumulation coincided with the signal of the macrophage marker, suggesting that the dysfunction of lysosomes in macrophages would result in a high susceptibility of Neu1-KO to E. piscicida. Chloroquine, an inhibitor of lysosomal degradation, induced high mortality of wild type zebrafish with E. piscicida infection accompanied by increased lysosomal accumulation, similar to Neu1-KO zebrafish. This study revealed that Neu1 sialidase plays a crucial role in the lysosomal degradation of macrophages with a bacterial infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping