PUBLICATION
Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo
- Authors
- Krishnan, N., Swoger, M., Rathbun, L.I., Fioramonti, P.J., Freshour, J., Bates, M., Patteson, A.E., Hehnly, H.
- ID
- ZDB-PUB-220320-6
- Date
- 2022
- Source
- Life science alliance 5(7): (Journal)
- Registered Authors
- Hehnly, Heidi
- Keywords
- none
- MeSH Terms
-
- Animals
- Antigens
- Centrosome/metabolism
- Endosomes/metabolism
- Zebrafish*
- rab GTP-Binding Proteins*/genetics
- rab GTP-Binding Proteins*/metabolism
- PubMed
- 35304423 Full text @ Life Sci Alliance
Citation
Krishnan, N., Swoger, M., Rathbun, L.I., Fioramonti, P.J., Freshour, J., Bates, M., Patteson, A.E., Hehnly, H. (2022) Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. Life science alliance. 5(7):.
Abstract
The last stage of cell division involves two daughter cells remaining interconnected by a cytokinetic bridge that is cleaved during abscission. Conserved between the zebrafish embryo and human cells, we found that the oldest centrosome moves in a Rab11-dependent manner towards the cytokinetic bridge sometimes followed by the youngest. Rab11-endosomes are organized in a Rab11-GTP dependent manner at the mother centriole during pre-abscission, with Rab11 endosomes at the oldest centrosome being more mobile compared with the youngest. The GTPase activity of Rab11 is necessary for the centrosome protein, Pericentrin, to be enriched at the centrosome. Reduction in Pericentrin expression or optogenetic disruption of Rab11-endosome function inhibited both centrosome movement towards the cytokinetic bridge and abscission, resulting in daughter cells prone to being binucleated and/or having supernumerary centrosomes. These studies suggest that Rab11-endosomes contribute to centrosome function during pre-abscission by regulating Pericentrin organization resulting in appropriate centrosome movement towards the cytokinetic bridge and subsequent abscission.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping