PUBLICATION
Lifelong single-cell profiling of cranial neural crest diversification in zebrafish
- Authors
- Fabian, P., Tseng, K.C., Thiruppathy, M., Arata, C., Chen, H.J., Smeeton, J., Nelson, N., Crump, J.G.
- ID
- ZDB-PUB-220112-7
- Date
- 2022
- Source
- Nature communications 13: 13 (Journal)
- Registered Authors
- Crump, Gage DeKoeyer, Smeeton, Joanna
- Keywords
- none
- Datasets
- GEO:GSE178969
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Chromatin
- Gene Expression Regulation, Developmental
- Neural Crest*/cytology
- Neural Crest*/metabolism
- Single-Cell Analysis*/methods
- Skull/cytology
- Transcriptome
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 35013168 Full text @ Nat. Commun.
Citation
Fabian, P., Tseng, K.C., Thiruppathy, M., Arata, C., Chen, H.J., Smeeton, J., Nelson, N., Crump, J.G. (2022) Lifelong single-cell profiling of cranial neural crest diversification in zebrafish. Nature communications. 13:13.
Abstract
The cranial neural crest generates a huge diversity of derivatives, including the bulk of connective and skeletal tissues of the vertebrate head. How neural crest cells acquire such extraordinary lineage potential remains unresolved. By integrating single-cell transcriptome and chromatin accessibility profiles of cranial neural crest-derived cells across the zebrafish lifetime, we observe progressive and region-specific establishment of enhancer accessibility for distinct fates. Neural crest-derived cells rapidly diversify into specialized progenitors, including multipotent skeletal progenitors, stromal cells with a regenerative signature, fibroblasts with a unique metabolic signature linked to skeletal integrity, and gill-specific progenitors generating cell types for respiration. By retrogradely mapping the emergence of lineage-specific chromatin accessibility, we identify a wealth of candidate lineage-priming factors, including a Gata3 regulatory circuit for respiratory cell fates. Rather than multilineage potential being established during cranial neural crest specification, our findings support progressive and region-specific chromatin remodeling underlying acquisition of diverse potential.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping