PUBLICATION
An enhancer trap zebrafish line for lateral line development and regulation of six2b expression
- Authors
- Fan, C., Ouyang, Y., Yuan, X., Wang, J.
- ID
- ZDB-PUB-220108-8
- Date
- 2022
- Source
- Gene expression patterns : GEP 43: 119231 (Journal)
- Registered Authors
- Fan, Chunxin
- Keywords
- Accessory cells, Canal, Lateral line, Wnt, six2b
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Lateral Line System*/metabolism
- Organogenesis
- Regulatory Sequences, Nucleic Acid
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 34995793 Full text @ Gene Expr. Patterns
Citation
Fan, C., Ouyang, Y., Yuan, X., Wang, J. (2022) An enhancer trap zebrafish line for lateral line development and regulation of six2b expression. Gene expression patterns : GEP. 43:119231.
Abstract
Zebrafish lateral line system which is derived from neurogenic placodes has become a popular model for developmental biology since its formation involves cell migration, pattern formation, organogenesis, and hair cell regeneration. Transgenic lines play a crucial role in lateral line system study. Here, we identified an enhancer trap transgenic zebrafish line Et(gata2a:EGFP)189b (ET189b for short), which expressed enhanced green fluorescent protein (EGFP) in the pituitary, otic, and lateral line placodes and their derivatives. Especially, in neuromast, the accessory cells rather than hair cells were labeled by EGFP. Furthermore, we found the Tol2 transposon construct is integrated at the proximal upstream region of six2b gene locus. And EGFP expression of ET189b closely reflects the expression of endogenous six2b during development and after dkk1bover-expression. Taken together, our results indicated that ET189b is an ideal line for research on lateral line development and regulation ofsix2b expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping