PUBLICATION
ATP-sensitive potassium channels in zebrafish cardiac and vascular smooth muscle
- Authors
- Singareddy, S.S., Roessler, H.I., McClenaghan, C., Ikle, J., Tryon, R., van Haaften, G., Nichols, C.G.
- ID
- ZDB-PUB-211129-27
- Date
- 2021
- Source
- The Journal of physiology 600(2): 299-312 (Journal)
- Registered Authors
- Nichols, Colin G., Singareddy, Soma, Tryon, Robert
- Keywords
- ABCC9, KATP, KCNJ8, Kir6.2, SUR2, cantú syndrome, cardiomyocytes, cardiovascular, ion channels, vascular smooth muscle cells, zebrafish
- MeSH Terms
-
- Animals
- Humans
- Hypertrichosis*
- KATP Channels*/genetics
- Muscle, Smooth, Vascular
- Myocytes, Cardiac
- Sulfonylurea Receptors/genetics
- Zebrafish
- PubMed
- 34820842 Full text @ J. Physiol.
Citation
Singareddy, S.S., Roessler, H.I., McClenaghan, C., Ikle, J., Tryon, R., van Haaften, G., Nichols, C.G. (2021) ATP-sensitive potassium channels in zebrafish cardiac and vascular smooth muscle. The Journal of physiology. 600(2):299-312.
Abstract
Key points Zebrafish cardiac myocytes (CM) and vascular smooth muscle (VSM) express functional KATP channels of similar subunit composition, structure, and metabolic sensitivity to their mammalian counterparts. In contrast to mammalian cardiovascular KATP channels, zebrafish channels are insensitive to potassium channel opener drugs (pinacidil, minoxidil) in both chambers of the heart and in VSM. We provide a first characterization of the molecular properties of fish KATP channels and validate the use of such genetically modified fish as models of human Cantú Syndrome and ABCC9-related Intellectual disability and Myopathy Syndrome.
Abstract ATP-sensitive potassium channels (KATP channels) are hetero-octameric nucleotide-gated ion channels that couple cellular metabolism to excitability in various tissues. In the heart, KATP channels are activated during ischemia and potentially during adrenergic stimulation. In the vasculature, they are normally active at a low level, reducing vascular tone, but the ubiquitous nature of these channels leads to complex and poorly understood channelopathies as a result of gain- or loss-of-function mutations. Zebrafish (ZF) models of these channelopathies may provide insights to the link between molecular dysfunction and complex pathophysiology, but this requires understanding the tissue dependence of channel activity and subunit specificity. Thus far, direct analysis of ZF KATP expression and functional properties has only been performed in pancreatic β-cells. Using a comprehensive combination of genetically modified fish, electrophysiology, and gene expression analysis, we demonstrate that ZF cardiac myocytes (CM) and vascular smooth muscle (VSM) express functional KATP channels of similar subunit composition, structure, and metabolic sensitivity to their mammalian counterparts. However, in contrast to mammalian cardiovascular KATP channels, ZF channels are insensitive to potassium channel opener drugs (pinacidil, minoxidil) in both chambers of the heart and in VSM. The results provide a first characterization of the molecular properties of fish KATP channels and validate the use of such genetically modified fish as models of human Cantú Syndrome and ABCC9-related Intellectual disability and Myopathy Syndrome. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping