PUBLICATION
Dietary Seleno-l-Methionine Causes Alterations in Neurotransmitters, Ultrastructure of the Brain, and Behaviors in Zebrafish (Danio rerio)
- Authors
- Li, X., Liu, H., Li, D., Lei, H., Wei, X., Schlenk, D., Mu, J., Chen, H., Yan, B., Xie, L.
- ID
- ZDB-PUB-210908-2
- Date
- 2021
- Source
- Environmental science & technology 55: 11894-11905 (Journal)
- Registered Authors
- Keywords
- behavioral toxicity, dietary Se-Met, neurotransmitters, signal pathways, zebrafish
- MeSH Terms
-
- Animals
- Antioxidants
- Brain
- Neurotransmitter Agents
- Selenomethionine
- Water Pollutants, Chemical*
- Zebrafish*
- PubMed
- 34488355 Full text @ Env. Sci. Tech.
Citation
Li, X., Liu, H., Li, D., Lei, H., Wei, X., Schlenk, D., Mu, J., Chen, H., Yan, B., Xie, L. (2021) Dietary Seleno-l-Methionine Causes Alterations in Neurotransmitters, Ultrastructure of the Brain, and Behaviors in Zebrafish (Danio rerio). Environmental science & technology. 55:11894-11905.
Abstract
Elevated concentrations of dietary selenium (Se) cause abnormalities and extirpation of fish inhabiting in Se-contaminated environments. However, its effect on fish behavior and the underlying mechanisms remain largely unknown. In this study, two-month-old zebrafish (Danio rerio) was fed seleno-l-methionine (Se-Met) at environmentally relevant concentrations (i.e., control (2.61), low (5.43), medium (12.16), and high (34.61) μg Se/g dry weight (dw), respectively, corresponding to the C, L, M, and H treatments) for 60 days. Targeted metabolomics, histopathological, and targeted transcriptional endpoints were compared to behavioral metrics to evaluate the effects of dietary exposure to Se-Met . The results showed that the levels of total Se and malondialdehyde in fish brains were increased in a dose-dependent pattern. Meanwhile, mitochondrial damages and decreased activities of the mitochondria respiratory chain complexes were observed in the neurons at the M and H treatments. In addition, dietary Se-Met affected neurotransmitters, metabolites, and transcripts of the genes associated with the dopamine, serotonin, gamma-aminobutyric acid, acetylcholine, and histamine signaling pathways in zebrafish brains at the H treatments. The total swimming distance and duration in the Novel Arm were lowered in fish from the H treatment. This study has demonstrated that dietary Se-Met affects the ultrastructure of the zebrafish brain, neurotransmitters, and associated fish behaviors and may help enhance adverse outcome pathways for neurotransmitter-behavior key events in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping