PUBLICATION

Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives

Authors
Lencer, E., Prekeris, R., Artinger, K.B.
ID
ZDB-PUB-210817-10
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Artinger, Kristin Bruk
Keywords
developmental biology, neural crest cells, pigment development, rohon-beard cells, scRNA-seq, xanthophores, zebrafish
Datasets
GEO:GSE163907
MeSH Terms
  • Animals
  • Cell Lineage
  • Cell Movement*
  • Embryo, Nonmammalian
  • Gene Expression
  • Genetic Markers*
  • Neural Crest/physiology*
  • Neurons/physiology
  • Sequence Analysis, RNA*
  • Single-Cell Analysis*
  • Zebrafish/embryology*
PubMed
34397384 Full text @ Elife
Abstract
The neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here, we use single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. We show that neural crest cells are transcriptionally diverse and identify pre-migratory populations already expressing genes associated with differentiated derivatives, specifically in the xanthophore lineage. Further, we identify a population of Rohon-Beard neurons in the data. The data presented identify novel genetic markers for multiple trunk neural crest cell populations and Rohon-Beard neurons providing insight into previously uncharacterized genes critical for vertebrate development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping