PUBLICATION
Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage
- Authors
- Houseright, R.A., Miskolci, V., Mulvaney, O., Bortnov, V., Mosher, D.F., Rindy, J., Bennin, D.A., Huttenlocher, A.
- ID
- ZDB-PUB-210529-11
- Date
- 2021
- Source
- The Journal of cell biology 220(8): (Journal)
- Registered Authors
- Huttenlocher, Anna
- Keywords
- none
- MeSH Terms
-
- Animal Fins/injuries
- Animal Fins/metabolism*
- Animal Fins/microbiology
- Animal Fins/pathology
- Animals
- Animals, Genetically Modified
- Cell Movement*
- Disease Models, Animal
- Hypoxia-Inducible Factor 1, alpha Subunit/genetics
- Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
- Inflammation/genetics
- Inflammation/metabolism*
- Inflammation/microbiology
- Macrophages/metabolism
- Macrophages/microbiology
- Microscopy, Fluorescence
- Neutrophil Infiltration*
- Neutrophils/metabolism*
- Neutrophils/microbiology
- Paracrine Communication
- Pseudomonas aeruginosa/pathogenicity
- Signal Transduction
- Time Factors
- Wound Healing*
- Wound Infection/genetics
- Wound Infection/metabolism*
- Wound Infection/microbiology
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 34047769 Full text @ J. Cell Biol.
Citation
Houseright, R.A., Miskolci, V., Mulvaney, O., Bortnov, V., Mosher, D.F., Rindy, J., Bennin, D.A., Huttenlocher, A. (2021) Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage. The Journal of cell biology. 220(8):.
Abstract
Neutrophil recruitment to tissue damage is essential for host defense but can also impede tissue repair. The cues that differentially regulate neutrophil responses to tissue damage and infection remain unclear. Here, we report that the paracrine factor myeloid-derived growth factor (MYDGF) is induced by tissue damage and regulates neutrophil motility to damaged, but not infected, tissues in zebrafish larvae. Depletion of MYDGF impairs wound healing, and this phenotype is rescued by depleting neutrophils. Live imaging and photoconversion reveal impaired neutrophil reverse migration and inflammation resolution in mydgf mutants. We found that persistent neutrophil inflammation in tissues of mydgf mutants was dependent on the HIF-1α pathway. Taken together, our data suggest that MYDGF is a damage signal that regulates neutrophil interstitial motility and inflammation through a HIF-1α pathway in response to tissue damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping