PUBLICATION
Stabilin-1 is required for the endothelial clearance of small anionic nanoparticles
- Authors
- Arias-Alpizar, G., Koch, B., Hamelmann, N.M., Neustrup, M.A., Paulusse, J.M.J., Jiskoot, W., Kros, A., Bussmann, J.
- ID
- ZDB-PUB-210411-8
- Date
- 2021
- Source
- Nanomedicine : nanotechnology, biology, and medicine 34: 102395 (Journal)
- Registered Authors
- Bussmann, Jeroen, Koch, Bjorn
- Keywords
- lipopolysaccharide, liver endothelium, nanoparticles, stabilin, zebrafish
- MeSH Terms
-
- Animals
- Anions
- Calcium-Binding Proteins/genetics
- Calcium-Binding Proteins/physiology*
- Endothelium/metabolism*
- Gene Knockdown Techniques
- Nanoparticles*
- Zebrafish/embryology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 33838334 Full text @ Nanomedicine
Citation
Arias-Alpizar, G., Koch, B., Hamelmann, N.M., Neustrup, M.A., Paulusse, J.M.J., Jiskoot, W., Kros, A., Bussmann, J. (2021) Stabilin-1 is required for the endothelial clearance of small anionic nanoparticles. Nanomedicine : nanotechnology, biology, and medicine. 34:102395.
Abstract
Clearance of nanoparticles (NPs) after intravenous injection - mainly by the liver - is a critical barrier for the clinical translation of nanomaterials. Physicochemical properties of NPs are known to influence their distribution through cell-specific interactions; however, the molecular mechanisms responsible for liver cellular NP uptake are poorly understood. Liver sinusoidal endothelial cells and Kupffer cells are critical participants in this clearance process. Here we use a zebrafish model for liver-NP interaction to identify the endothelial scavenger receptor Stabilin-1 as a non-redundant receptor for the clearance of small anionic NPs. Furthermore, we show that physiologically, Stabilin-1 is required for the removal of bacterial lipopolysaccharide (LPS/endotoxin) from circulation and that Stabilin-1 cooperates with its homolog Stabilin-2 in the clearance of larger (~100 nm) anionic NPs. Our findings allow optimization of anionic nanomedicine biodistribution and targeting therapies that use Stabilin-1 and -2 for liver endothelium-specific delivery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping