PUBLICATION
The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium
- Authors
- Willoughby, P.M., Allen, M., Yu, J., Korytnikov, R., Chen, T., Liu, Y., So, I., Macpherson, N., Mitchell, J.A., Fernandez-Gonzalez, R., Bruce, A.E.E.
- ID
- ZDB-PUB-210324-8
- Date
- 2021
- Source
- eLIFE 10: (Journal)
- Registered Authors
- Bruce, Ashley
- Keywords
- cell biology, developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Cell Movement/genetics*
- Cytokinesis
- Embryo, Nonmammalian/physiology
- Epithelium/physiology
- Gastrula/physiology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins
- rab GTP-Binding Proteins/genetics*
- rab GTP-Binding Proteins/metabolism
- PubMed
- 33755014 Full text @ Elife
Citation
Willoughby, P.M., Allen, M., Yu, J., Korytnikov, R., Chen, T., Liu, Y., So, I., Macpherson, N., Mitchell, J.A., Fernandez-Gonzalez, R., Bruce, A.E.E. (2021) The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium. eLIFE. 10:.
Abstract
In emerging epithelial tissues, cells undergo dramatic rearrangements to promote tissue shape changes. Dividing cells remain interconnected via transient cytokinetic bridges. Bridges are cleaved during abscission and currently, the consequences of disrupting abscission in developing epithelia are not well understood. We show that the Rab GTPase, Rab25, localizes near cytokinetic midbodies and likely coordinates abscission through endomembrane trafficking in the epithelium of the zebrafish gastrula during epiboly. In maternal-zygotic Rab25a and Rab25b mutant embryos, morphogenic activity tears open persistent apical cytokinetic bridges that failed to undergo timely abscission. Cytokinesis defects result in anisotropic cell morphologies that are associated with a reduction of contractile actomyosin networks. This slows cell rearrangements and alters the viscoelastic responses of the tissue, all of which likely contribute to delayed epiboly. We present a model in which Rab25 trafficking coordinates cytokinetic bridge abscission and cortical actin density, impacting local cell shape changes and tissue-scale forces.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping