PUBLICATION
Phylogenetic and developmental analyses indicate complex functions of Calcium-Activated Potassium Channels in zebrafish embryonic development
- Authors
- Silic, M.R., Black, M.M., Zhang, G.
- ID
- ZDB-PUB-210319-4
- Date
- 2021
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 250(10): 1477-1493 (Journal)
- Registered Authors
- Zhang, GuangJun
- Keywords
- Calcium-activated Potassium Ion channels, KCNMA, KCNMB, KCNN, KCNT, KCa channels, in situ hybridization, phylogeny, whole genome duplication (WGD), zebrafish
- MeSH Terms
-
- Animals
- Embryonic Development/physiology*
- Gene Expression Regulation, Developmental
- Phylogeny*
- Potassium Channels, Calcium-Activated/genetics
- Potassium Channels, Calcium-Activated/metabolism*
- Somites/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 33728688 Full text @ Dev. Dyn.
Citation
Silic, M.R., Black, M.M., Zhang, G. (2021) Phylogenetic and developmental analyses indicate complex functions of Calcium-Activated Potassium Channels in zebrafish embryonic development. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(10):1477-1493.
Abstract
Background Calcium-activated potassium channels (KCa) are a specific type of potassium channel activated by intracellular calcium concentration changes. This group of potassium channels plays fundamental roles ranging from regulating neuronal excitability to immune cell activation. Many human diseases such as schizophrenia, hypertension, epilepsy, and cancers have been linked to mutations in this group of potassium channels. Although the KCa channels have been extensively studied electrophysiologically and pharmacologically, their spatiotemporal gene expression during embryogenesis remains mostly unknown.
Results Using zebrafish as a model, we identified and renamed 14 KCa genes. We further performed phylogenetic and syntenic analyses on vertebrate KCa genes. Our data revealed that the number of KCa genes in zebrafish was increased, most likely due to teleost-specific whole-genome duplication. Moreover, we examined zebrafish KCa gene expression during early embryogenesis. The duplicated ohnologous genes show distinct and overlapped gene expression. Furthermore, we found that zebrafish KCa genes are expressed in various tissues and organs (somites, fins, olfactory regions, eye, kidney, etc.) and neuronal tissues, suggesting that they may play important roles during zebrafish embryogenesis.
Conclusions Our phylogenetic and developmental analyses shed light on the potential functions of the KCa genes during embryogenesis related to congenital diseases and human channelopathies. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping