PUBLICATION
Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube
- Authors
- Symonds, A.C., Buckley, C.E., Williams, C.A., Clarke, J.D.W.
- ID
- ZDB-PUB-201229-32
- Date
- 2020
- Source
- Development (Cambridge, England) 147(24): (Journal)
- Registered Authors
- Clarke, Jon
- Keywords
- Adhesions, Epithelial tube, Morphogenesis, Neural tube, Zebrafish
- MeSH Terms
-
- Animals
- Cell Polarity/genetics
- Epithelial Cells/metabolism
- Gene Expression Regulation, Developmental/genetics
- Guanylate Cyclase/genetics*
- Intercellular Junctions/genetics
- Membrane Proteins
- Morphogenesis/genetics*
- Neural Tube/growth & development
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics*
- rab GTP-Binding Proteins/genetics*
- PubMed
- 33361092 Full text @ Development
Citation
Symonds, A.C., Buckley, C.E., Williams, C.A., Clarke, J.D.W. (2020) Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube. Development (Cambridge, England). 147(24):.
Abstract
Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping