PUBLICATION
A csf1rb mutation uncouples two waves of microglia development in zebrafish
- Authors
- Ferrero, G., Miserocchi, M., Di Ruggiero, E., Wittamer, V.
- ID
- ZDB-PUB-201212-14
- Date
- 2020
- Source
- Development (Cambridge, England) 148(1): (Journal)
- Registered Authors
- Wittamer, Valerie
- Keywords
- Colony-stimulating factor 1 receptor, Development, Hematopoiesis, Macrophages, Microglia, Zebrafish
- MeSH Terms
-
- Aging/genetics
- Animals
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Gene Expression Regulation, Developmental
- Hematopoiesis
- Hematopoietic Stem Cells/metabolism
- Macrophages/metabolism
- Microglia/metabolism*
- Mutation/genetics*
- Myeloid Cells/metabolism
- Phagocytes/metabolism
- Protein-Tyrosine Kinases/genetics*
- Protein-Tyrosine Kinases/metabolism
- Receptor Protein-Tyrosine Kinases/genetics
- Receptor Protein-Tyrosine Kinases/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 33298459 Full text @ Development
Citation
Ferrero, G., Miserocchi, M., Di Ruggiero, E., Wittamer, V. (2020) A csf1rb mutation uncouples two waves of microglia development in zebrafish. Development (Cambridge, England). 148(1):.
Abstract
In vertebrates, the ontogeny of microglia, the resident macrophages of the central nervous system, initiates early during development from primitive macrophages. While murine embryonic microglia then persist through life, in zebrafish these cells are transient, as they are fully replaced by an adult population originating from larval hematopoietic stem cell (HSC)-derived progenitors. Colony-stimulating factor receptor 1 (csf1r) is a fundamental regulator of microglia ontogeny in vertebrates, including zebrafish which possess two paralogous genes: csf1ra and csf1rb While previous work showed mutation in both genes completely abrogates microglia development, the specific contribution of each paralog remains largely unknown. Here, using a fate-mapping strategy to discriminate between the two microglial waves, we uncover non-overlapping roles for csf1ra and csf1rb in hematopoiesis, and identified csf1rb as an essential regulator of adult microglia development. Notably, we demonstrate that csf1rb positively regulates HSC-derived myelopoiesis, resulting in macrophage deficiency, including microglia, in adult mutant animals. Overall, this study contributes to new insights into evolutionary aspects of Csf1r signaling and provides an unprecedented framework for the functional dissection of embryonic versus adult microglia in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping