PUBLICATION
Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens
- Authors
- Hao, Y., Zhou, Y., Yu, Y., Zheng, M., Weng, K., Kou, Z., Liang, J., Zhang, Q., Tang, X., Xu, P., Link, B.A., Yao, K., Zou, J.
- ID
- ZDB-PUB-201020-24
- Date
- 2020
- Source
- Development (Cambridge, England) 147(22): (Journal)
- Registered Authors
- Keywords
- Adherens junctions remodeling, Apical polarity, MPP5a, Rab11, Zonula adherens
- MeSH Terms
-
- Adherens Junctions/genetics
- Adherens Junctions/metabolism*
- Animals
- Cadherins/genetics
- Cadherins/metabolism
- Cell Movement/physiology*
- Cell Polarity/physiology*
- Epithelial Cells
- Golgi Apparatus/genetics
- Golgi Apparatus/metabolism
- Guanylate Cyclase/genetics
- Guanylate Cyclase/metabolism*
- Protein Transport/physiology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism*
- PubMed
- 33060129 Full text @ Development
Citation
Hao, Y., Zhou, Y., Yu, Y., Zheng, M., Weng, K., Kou, Z., Liang, J., Zhang, Q., Tang, X., Xu, P., Link, B.A., Yao, K., Zou, J. (2020) Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens. Development (Cambridge, England). 147(22):.
Abstract
Adherens junctions remodeling regulated by apical polarity proteins constitutes a major driving force for tissue morphogenesis, though the precise mechanism remains inconclusive. Here we reported that Crumbs complex component MPP5a interacts with small GTPase Rab11 in Golgi to synergistically transport cadherin and Crumbs components to the apical domain, thus establishing the apical epithelial polarity and adherens junctions. In contrast, Par complex recruited by MPP5a is incapable to interact with Rab11 but may assemble cytoskeleton to facilitate the cadherin exocytosis. In accordance, dysfunction of MPP5a induced an invasive migration of epithelial cells. This adherens junctions remodeling pattern is frequently observed in zebrafish lens epithelial cells and neuroepithelial cells. The data identified an unrecognized MPP5a/Rab11 complex and described its essential role in guiding the apical polarization and zonula adherens formation in epithelial cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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