PUBLICATION
Zebrafish hif-3α modulates erythropoiesis via regulation of gata-1 to facilitate hypoxia tolerance
- Authors
- Cai, X., Zhou, Z., Zhu, J., Liao, Q., Zhang, D., Liu, X., Wang, J., Ouyang, G., Xiao, W.
- ID
- ZDB-PUB-201011-9
- Date
- 2020
- Source
- Development (Cambridge, England) 147(22): (Journal)
- Registered Authors
- Keywords
- Erythropoiesis, Gata-1, Hif-3α, Hypoxia, Zebrafish
- MeSH Terms
-
- Animals
- Antigens, Differentiation/biosynthesis
- Antigens, Differentiation/genetics
- Apoptosis Regulatory Proteins/genetics
- Apoptosis Regulatory Proteins/metabolism*
- Down-Regulation
- Erythrocytes/metabolism*
- Erythrocytes/pathology
- Erythropoiesis*
- GATA1 Transcription Factor/genetics
- GATA1 Transcription Factor/metabolism*
- Hypoxia/genetics
- Hypoxia/metabolism*
- Hypoxia/pathology
- Response Elements
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33037038 Full text @ Development
Citation
Cai, X., Zhou, Z., Zhu, J., Liao, Q., Zhang, D., Liu, X., Wang, J., Ouyang, G., Xiao, W. (2020) Zebrafish hif-3α modulates erythropoiesis via regulation of gata-1 to facilitate hypoxia tolerance. Development (Cambridge, England). 147(22):.
Abstract
The hypoxia-inducible factors 1α and 2α (HIF-1α and HIF-2α) are master regulators of the cellular response to O2. In addition to HIF-1α and HIF-2α, HIF-3α is another identified member of the HIF-α gene family. Even though whether some HIF-3α isoforms have transcriptional activity or repressive activity is still under debate, it is evident that the full length of HIF-3α acts as a transcription factor. However, its function in hypoxia signaling is largely unknown. Here, we showed that loss of hif-3α in zebrafish reduced hypoxia tolerance. Further assays indicated that erythrocyte number was decreased because red blood cell maturation was impeded by hif-3α disruption. We found that gata-1 expression was downregulated in hif-3α-null zebrafish, as were several hematopoietic marker genes, including alas2, band3, hbae1, hbae3 and hbbe1hif-3α recognized the hypoxia response element (HRE) located in the promoter of gata-1 and directly bound to the promoter to transactivate gata-1 expression. Our results suggested that hif-3α facilities hypoxia tolerance by modulating erythropoiesis via gata-1 regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping