PUBLICATION
Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer
- Authors
- Barske, L., Fabian, P., Hirschberger, C., Jandzik, D., Square, T., Xu, P., Nelson, N., Yu, H.V., Medeiros, D.M., Gillis, J.A., Crump, J.G.
- ID
- ZDB-PUB-201002-113
- Date
- 2020
- Source
- Proceedings of the National Academy of Sciences of the United States of America 117(40): 24876-24884 (Journal)
- Registered Authors
- Barske, Lindsey, Medeiros, Daniel, Xu, Pengfei
- Keywords
- Pou3f3, enhancer deletion, gill cover, neural crest, vertebrate evolution
- Datasets
- GEO:GSE140636
- MeSH Terms
-
- Animals
- Enhancer Elements, Genetic*
- Evolution, Molecular*
- Fishes/classification
- Fishes/genetics
- Fishes/growth & development
- Gills/growth & development*
- Mutation
- POU Domain Factors/genetics*
- Phylogeny
- Sharks/classification
- Sharks/genetics
- Sharks/growth & development
- Vertebrates/classification
- Vertebrates/genetics*
- Vertebrates/growth & development
- PubMed
- 32958671 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Barske, L., Fabian, P., Hirschberger, C., Jandzik, D., Square, T., Xu, P., Nelson, N., Yu, H.V., Medeiros, D.M., Gillis, J.A., Crump, J.G. (2020) Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer. Proceedings of the National Academy of Sciences of the United States of America. 117(40):24876-24884.
Abstract
Whereas the gill chambers of jawless vertebrates open directly into the environment, jawed vertebrates evolved skeletal appendages that drive oxygenated water unidirectionally over the gills. A major anatomical difference between the two jawed vertebrate lineages is the presence of a single large gill cover in bony fishes versus separate covers for each gill chamber in cartilaginous fishes. Here, we find that these divergent patterns correlate with the pharyngeal arch expression of Pou3f3 orthologs. We identify a deeply conserved Pou3f3 arch enhancer present in humans through sharks but undetectable in jawless fish. Minor differences between the bony and cartilaginous fish enhancers account for their restricted versus pan-arch expression patterns. In zebrafish, mutation of Pou3f3 or the conserved enhancer disrupts gill cover formation, whereas ectopic pan-arch Pou3f3b expression generates ectopic skeletal elements resembling the multimeric covers of cartilaginous fishes. Emergence of this Pou3f3 arch enhancer >430 Mya and subsequent modifications may thus have contributed to the acquisition and diversification of gill covers and respiratory strategies during gnathostome evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping