PUBLICATION
Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae
- Authors
- Gupta, R.K., Wasilewska, I., Palchevska, O., Kuźnicki, J.
- ID
- ZDB-PUB-200902-2
- Date
- 2020
- Source
- International Journal of Molecular Sciences 21(17): (Journal)
- Registered Authors
- Gupta, Rishikesh Kumar, Wasilewska, Iga
- Keywords
- PTZ, SOCE, Stim2a, behavioral tests, calcium toolkit, glutamate, hyperactivity, zebrafish
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Calcium Signaling
- Disease Models, Animal
- Gene Expression Profiling
- Gene Knockout Techniques
- Glutamic Acid/pharmacology
- Hyperkinesis/genetics*
- Hyperkinesis/metabolism
- Larva/genetics
- Neurons/metabolism*
- Pentylenetetrazole/pharmacology
- Phenotype
- Phototaxis/drug effects
- Sequence Analysis, RNA
- Stromal Interaction Molecule 2/genetics*
- Transcription Factors/genetics*
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 32867296 Full text @ Int. J. Mol. Sci.
Citation
Gupta, R.K., Wasilewska, I., Palchevska, O., Kuźnicki, J. (2020) Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae. International Journal of Molecular Sciences. 21(17):.
Abstract
Stromal interaction molecule (STIM) proteins play a crucial role in store-operated calcium entry (SOCE) as endoplasmic reticulum Ca2+ sensors. In neurons, STIM2 was shown to have distinct functions from STIM1. However, its role in brain activity and behavior was not fully elucidated. The present study analyzed behavior in zebrafish (Danio rerio) that lacked stim2a. The mutant animals had no morphological abnormalities and were fertile. RNA-sequencing revealed alterations of the expression of transcription factor genes and several members of the calcium toolkit. Neuronal Ca2+ activity was measured in vivo in neurons that expressed the GCaMP5G sensor. Optic tectum neurons in stim2a-/- fish had more frequent Ca2+ signal oscillations compared with neurons in wildtype (WT) fish. We detected an increase in activity during the visual-motor response test, an increase in thigmotaxis in the open field test, and the disruption of phototaxis in the dark/light preference test in stim2a-/- mutants compared with WT. Both groups of animals reacted to glutamate and pentylenetetrazol with an increase in activity during the visual-motor response test, with no major differences between groups. Altogether, our results suggest that the hyperactive-like phenotype of stim2a-/- mutant zebrafish is caused by the dysregulation of Ca2+ homeostasis and signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping