PUBLICATION
Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes
- Authors
- Wang, M., Du, L., Lee, A.C., Li, Y., Qin, H., He, J.
- ID
- ZDB-PUB-200724-21
- Date
- 2020
- Source
- The Journal of cell biology 219(9): (Journal)
- Registered Authors
- Du, Lei, He, Jie, Li, Yan, Qin, Huiwen, Wang, Mei
- Keywords
- none
- Datasets
- GEO:GSE150839
- MeSH Terms
-
- Amacrine Cells/physiology
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology
- Cell Lineage/physiology*
- Chromatin/physiology
- Gene Expression Regulation, Developmental/physiology
- Neurogenesis/physiology
- Retina/physiology*
- Retinal Neurons/physiology*
- Zebrafish/physiology
- PubMed
- 32699896 Full text @ J. Cell Biol.
Citation
Wang, M., Du, L., Lee, A.C., Li, Y., Qin, H., He, J. (2020) Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes. The Journal of cell biology. 219(9):.
Abstract
How astounding neuronal diversity arises from variable cell lineages in vertebrates remains mostly elusive. By in vivo lineage tracing of ∼1,000 single zebrafish retinal progenitors, we identified a repertoire of subtype-specific stereotyped neurogenic lineages. Remarkably, within these stereotyped lineages, GABAergic amacrine cells were born with photoreceptor cells, whereas glycinergic amacrine cells were born with OFF bipolar cells. More interestingly, post-mitotic differentiation blockage of GABAergic and glycinergic amacrine cells resulted in their respecification into photoreceptor and bipolar cells, respectively, suggesting lineage constraint in cell subtype specification. Using single-cell RNA-seq and ATAC-seq analyses, we further identified lineage-specific progenitors, each defined by specific transcription factors that exhibited characteristic chromatin accessibility dynamics. Finally, single pro-neural factors could specify different neuron types/subtypes in a lineage-dependent manner. Our findings reveal the importance of lineage context in defining neuronal subtypes and provide a demonstration of in vivo lineage-dependent induction of unique retinal neuron subtypes for treatment purposes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping