PUBLICATION
A CRISPR Mutagenesis Screen of Ctnnd1
- Authors
- Thyme, S.B., and Carte, A.
- ID
- ZDB-PUB-200621-10
- Date
- 2020
- Source
- ZebraShare : (Unpublished)
- Registered Authors
- Thyme, Summer
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Thyme, S.B., and Carte, A. (2020) A CRISPR Mutagenesis Screen of Ctnnd1. ZebraShare. .
Abstract
CRISPRs were used to generate a mutant in this gene in exon 5. The generated allele is a 31 base-pair deletion (see attached image of alignment of with CRISPRs (teal) and genotyping primers (pink)). The mutant is dying between 7-8 somite and 24 hpf. Attached pictures were taken blind and then the mutants and controls were genotyped.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping