PUBLICATION
Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio)
- Authors
- Shiozaki, K., Kawabe, M., Karasuyama, K., Kurachi, T., Hayashi, A., Ataka, K., Iwai, H., Takeno, H., Hayasaka, O., Kotani, T., Komatsu, M., Inui, A.
- ID
- ZDB-PUB-200422-23
- Date
- 2020
- Source
- Scientific Reports 10: 5913 (Journal)
- Registered Authors
- Shiozaki, Kazuhiro
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Anxiety/genetics*
- Anxiety/physiopathology
- Anxiety/psychology
- Behavior, Animal/physiology
- Catecholamines/metabolism
- Disease Models, Animal
- Energy Metabolism/physiology*
- Female
- Gene Knockout Techniques
- Humans
- Male
- Neuropeptide Y/deficiency*
- Neuropeptide Y/genetics
- Orexins/metabolism
- Receptors, Neuropeptide Y/metabolism
- Signal Transduction/physiology
- Stress, Psychological/physiopathology*
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 32246073 Full text @ Sci. Rep.
Citation
Shiozaki, K., Kawabe, M., Karasuyama, K., Kurachi, T., Hayashi, A., Ataka, K., Iwai, H., Takeno, H., Hayasaka, O., Kotani, T., Komatsu, M., Inui, A. (2020) Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio). Scientific Reports. 10:5913.
Abstract
Neuropeptide Y (NPY) controls energy homeostasis including orexigenic actions in mammalians and non-mammalians. Recently, NPY has attracted attention as a mediator of emotional behaviour and psychosomatic diseases. However, its functions are not fully understood. We established npy gene-deficient (NPY-KO) zebrafish (Danio rerio) to assess the relationship between NPY and emotional behaviours. The NPY-KO zebrafish exhibited similar growth, but pomc and avp mRNA levels in the brain were higher as compared to wild-type fish. NPY-KO zebrafish exhibited several anxiety-like behaviours, such as a decrease in social interaction in mirror test and decreased locomotion in black-white test. The acute cold stress-treated NPY-KO zebrafish exhibited anxiety-like behaviours such as remaining stationary and swimming along the side of the tank in the mirror test. Moreover, expression levels of anxiety-associated genes (orx and cck) and catecholamine production (gr, mr, th1 and th2) were significantly higher in NPY-KO zebrafish than in wild-type fish. We demonstrated that NPY-KO zebrafish have an anxiety phenotype and a stress-vulnerability like NPY-KO mice, whereby orx and/or catecholamine signalling may be involved in the mechanism actions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping