PUBLICATION
Cytokinetic bridge triggers de novo lumen formation in vivo
- Authors
- Rathbun, L.I., Colicino, E.G., Manikas, J., O'Connell, J., Krishnan, N., Reilly, N.S., Coyne, S., Erdemci-Tandogan, G., Garrastegui, A., Freshour, J., Santra, P., Manning, M.L., Amack, J.D., Hehnly, H.
- ID
- ZDB-PUB-200403-17
- Date
- 2020
- Source
- Nature communications 11: 1269 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Division/physiology*
- Cell Line
- Cell Movement
- Cell Polarity/physiology*
- Cilia/physiology
- Cystic Fibrosis Transmembrane Conductance Regulator/metabolism
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology*
- Embryonic Development/genetics
- Embryonic Development/physiology*
- Epithelial Cells/metabolism
- Gene Expression Regulation, Developmental
- Kupffer Cells/cytology
- Kupffer Cells/physiology*
- Mitosis
- Organogenesis/physiology*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism
- PubMed
- 32152267 Full text @ Nat. Commun.
Citation
Rathbun, L.I., Colicino, E.G., Manikas, J., O'Connell, J., Krishnan, N., Reilly, N.S., Coyne, S., Erdemci-Tandogan, G., Garrastegui, A., Freshour, J., Santra, P., Manning, M.L., Amack, J.D., Hehnly, H. (2020) Cytokinetic bridge triggers de novo lumen formation in vivo. Nature communications. 11:1269.
Abstract
Multicellular rosettes are transient epithelial structures that serve as intermediates during diverse organ formation. We have identified a unique contributor to rosette formation in zebrafish Kupffer's vesicle (KV) that requires cell division, specifically the final stage of mitosis termed abscission. KV utilizes a rosette as a prerequisite before forming a lumen surrounded by ciliated epithelial cells. Our studies identify that KV-destined cells remain interconnected by cytokinetic bridges that position at the rosette's center. These bridges act as a landmark for directed Rab11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transmembrane conductance regulator). Here we report that premature bridge cleavage through laser ablation or inhibiting abscission using optogenetic clustering of Rab11 result in disrupted lumen formation. We present a model in which KV mitotic cells strategically place their cytokinetic bridges at the rosette center, where Rab11-associated vesicles transport CFTR to aid in lumen establishment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping