PUBLICATION
Zebrafish miR-462-731 is required for digestive organ development
- Authors
- Huang, Y., Huang, C.X., Wang, W.F., Liu, H., Wang, H.L.
- ID
- ZDB-PUB-200403-150
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Part D, Genomics & proteomics 34: 100679 (Journal)
- Registered Authors
- Keywords
- Digestive organ development, Dkk3b, Liver, MicroRNA, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental*
- MicroRNAs/genetics*
- Pancreas, Exocrine/embryology*
- Pancreas, Exocrine/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32200130 Full text @ Comp. Biochem. Physiol. D Genom. Prot.
Citation
Huang, Y., Huang, C.X., Wang, W.F., Liu, H., Wang, H.L. (2020) Zebrafish miR-462-731 is required for digestive organ development. Comparative biochemistry and physiology. Part D, Genomics & proteomics. 34:100679.
Abstract
MicroRNAs (miRNAs), as important regulators of post-transcriptional gene expression, play important roles in the occurrence and function of organs. In this study, morpholino (MO) knockdown of miR-462/miR-731 was used to investigate the potential mechanisms of the miR-462-731 cluster during zebrafish liver development. The results showed significant reduction of digestive organs, especially liver and exocrine pancreas after the miR-462/miR-731 knockdown, and those phenotypes could be partially rescued by corresponding miRNA duplex. Acinar cells of the exocrine pancreas were also severely affected with pancreatic insufficiency. In particular, knockdown of miR-462 caused pancreas morphogenesis abnormity with specific bilateral exocrine pancreas. Additionally, it was found that miR-731 played a role in liver and exocrine pancreas development by directly targeting dkk3b, instead of the down-regulation of Wnt/β-catenin signaling. These findings contribute significantly to our understanding of molecular mechanisms of miR-462-731 cluster in development of digestive organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping