PUBLICATION
Neuroprotective Effects of Mitoquinone and Oleandrin on Parkinson's Disease Model in Zebrafish
- Authors
- Ünal, İ., Çalışkan-Ak, E., Üstündağ, Ü.V., Ateş, P.S., Alturfan, A.A., Altinoz, M.A., Elmaci, I., Emekli-Alturfan, E.
- ID
- ZDB-PUB-191128-1
- Date
- 2019
- Source
- The International journal of neuroscience 130(6): 574-582 (Journal)
- Registered Authors
- Ateş, Perihan Seda, Emekli-Alturfan, Ebru, Üstündağ, Ünsal Veli
- Keywords
- Mitoquinone, Oleandrin, Parkinson’s Disease, Zebrafish
- MeSH Terms
-
- Animals
- Cardenolides/administration & dosage*
- Disease Models, Animal
- Female
- Fish Proteins/metabolism
- Gene Expression/drug effects*
- Locomotion/drug effects
- Male
- Mitochondria/drug effects
- Neuroprotective Agents/administration & dosage*
- Organophosphorus Compounds/administration & dosage*
- Parkinson Disease/metabolism*
- Parkinsonian Disorders/chemically induced
- Rotenone/administration & dosage
- Synucleins/metabolism
- Ubiquinone/administration & dosage
- Ubiquinone/analogs & derivatives*
- Zebrafish
- PubMed
- 31771386 Full text @ Int. J. Neurosci.
Citation
Ünal, İ., Çalışkan-Ak, E., Üstündağ, Ü.V., Ateş, P.S., Alturfan, A.A., Altinoz, M.A., Elmaci, I., Emekli-Alturfan, E. (2019) Neuroprotective Effects of Mitoquinone and Oleandrin on Parkinson's Disease Model in Zebrafish. The International journal of neuroscience. 130(6):574-582.
Abstract
The aim of this study is to investigate the possible protective effects of mitoquinone and oleandrin on rotenone induced Parkinson's disease in zebrafish. Adult zebrafish were exposed to rotenone and mitoquinone for 30 days. Biochemical parameters were determined by spectrophotometric method and Parkinson's disease-related gene expressions were determined by reverse transcription polymerase chain reaction method. Measurement of neurotransmitters was performed by liquid chromatography tandem-mass spectrometry instrument. The accumulation of synuclein was demonstrated by immunohistochemical staining. In vitro thiazolyl blue tetrazolium bromide method was applied to determine the mitochondrial function of synaptosomal brain fractions using rotenone as a neurotoxic agent and mitoquinone and oleandrin as neuroprotective agents. Mitoquinone improved the oxidant- antioxidant balance and neurotransmitter levels that were disrupted by rotenone. Mitoquinone also ameliorated the expressions of Parkinson's disease-related gene expressions that were disrupted by rotenone. According to thiazolyl blue tetrazolium bromide assay results, mitoquinone and oleandrin increased mitochondrial function which was decreased due to rotenone exposure. Based on the results of our study, positive effects of mitoquinone were observed in Parkinson's disease model induced by rotenone in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping