PUBLICATION
Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts
- Authors
- Peng, L., Zhou, Y., Xu, W., Jiang, M., Li, H., Long, M., Liu, W., Liu, J., Zhao, X., Xiao, Y.
- ID
- ZDB-PUB-191011-14
- Date
- 2019
- Source
- International journal of biological sciences 15: 2340-2349 (Journal)
- Registered Authors
- Keywords
- doxycycline, fibroblast, induced pluripotent stem cell, lentiviral delivery system, zebra fish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Line
- Doxycycline
- Fibroblasts/cytology*
- Induced Pluripotent Stem Cells/cytology*
- Transduction, Genetic
- Zebrafish/embryology
- PubMed
- 31595152 Full text @ Int. J. Biol. Sci.
Citation
Peng, L., Zhou, Y., Xu, W., Jiang, M., Li, H., Long, M., Liu, W., Liu, J., Zhao, X., Xiao, Y. (2019) Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts. International journal of biological sciences. 15:2340-2349.
Abstract
Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established for mammals, few attempts have been made to induce iPS cells in nonmammalian species. Because of technical advantages over other vertebrates on stem cells, induced pluripotent stem cells from fish could be of value for research. In this paper, stable iPS-like cell lines were generated from adult zebra fish fibroblasts by combining the doxycycline inducible lentiviral delivery system and chemical treatment. RT-PCR analysis, alkaline phosphatase staining, and immunofluorescence indicated that adult zebra fish fibroblasts were successfully reprogrammed into iPS-like cells (ziPSCs). The ziPSCs exhibited stable growth and manifested many features of fish embryonic stem cells with pluripotency in vitro and in vivo. Because of easy maintenance, the developed technology in this study for generating zebra fish iPS-like cells could be extended to investigating other genera of fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping