PUBLICATION
Notch1a can widely mediate innate immune responses in zebrafish larvae infected with Vibrio parahaemolyticus
- Authors
- Ji, C., Guo, X., Dong, X., Ren, J., Zu, Y., Li, W., Zhang, Q.
- ID
- ZDB-PUB-190705-8
- Date
- 2019
- Source
- Fish & shellfish immunology 92: 680-689 (Journal)
- Registered Authors
- Zu, Yao
- Keywords
- Innate immunity, Notch1a, Transcriptome, Vibrio parahaemolyticus, zebrafish
- MeSH Terms
-
- Animals
- Fish Diseases/immunology
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/immunology*
- Immunity, Innate/genetics*
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/immunology*
- Receptor, Notch1/genetics*
- Receptor, Notch1/immunology*
- Signal Transduction/immunology*
- Vibrio Infections/immunology
- Vibrio Infections/veterinary
- Vibrio parahaemolyticus/physiology
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/immunology*
- PubMed
- 31271837 Full text @ Fish Shellfish Immunol.
Citation
Ji, C., Guo, X., Dong, X., Ren, J., Zu, Y., Li, W., Zhang, Q. (2019) Notch1a can widely mediate innate immune responses in zebrafish larvae infected with Vibrio parahaemolyticus. Fish & shellfish immunology. 92:680-689.
Abstract
The Notch signaling pathway is known to regulate innate immunity by influencing macrophage function and interacting with the Toll-like receptor (TLR) signaling pathway. However, the comprehensive role of the Notch signaling pathway in the innate immune response remains unknown. To assess the function of Notch1a in immunity, we examined the innate immune responses to Vibrio parahaemolyticus strain Vp13 of wild-type (WT) and notch1a-/- zebrafish larvae generated using the clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system. The median lethal dose (LD50) of V. parahaemolyticus was significantly lower in notch1a-/- larvae than in WT larvae 3 days post fertilization (dpf). Transcriptome data analysis revealed 359 significantly differentially expressed genes (DEGs), including 246 significantly down-regulated genes and 113 significantly up-regulated genes, in WT infected groups compared with WT control groups. In contrast, 986 significantly DEGs were found in notch1a-/- infected groups compared with notch1a-/- control groups, of which 82 genes were significantly down-regulated and 904 genes were significantly up-regulated. These DEGs belonged to the tumor necrosis factor (TNF), complement, nuclear factor kappa B (NF-κB), cathepsin, interleukin (IL), chemokine, serpin peptidase inhibitor, matrix metallopeptidase, innate immune cells, pattern recognition receptor (PRR), and other cytokine families. Our results indicate that Notch1a plays roles in inhibiting many immunity-related genes and could comprehensively mediate the innate immune response by regulating TLRs, nucleotide-binding-oligomerization-domain-like receptors (NLRs), lectins, complement, ILs, chemokines, TNF, cathepsin, and serpin. Further studies are required to understand the specific mechanism of Notch1a in innate immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping