PUBLICATION

Fate plasticity and reprogramming in genetically distinct populations of Danio leucophores

Authors
Lewis, V.M., Saunders, L.M., Larson, T.A., Bain, E.J., Sturiale, S.L., Gur, D., Chowdhury, S., Flynn, J.D., Allen, M.C., Deheyn, D.D., Lee, J.C., Simon, J.A., Lippincott-Schwartz, J., Raible, D.W., Parichy, D.M.
ID
ZDB-PUB-190530-2
Date
2019
Source
Proceedings of the National Academy of Sciences of the United States of America   116(24): 11806-11811 (Journal)
Registered Authors
Gur, Dvir, Parichy, David M., Raible, David
Keywords
evolution, neural crest, pigmentation, transdifferentiation, zebrafish
Datasets
GEO:GSE130526
MeSH Terms
  • Animals
  • Cell Plasticity/genetics*
  • Embryo, Nonmammalian/physiology
  • Gene Expression Regulation, Developmental/genetics
  • Genetics, Population/methods
  • Melanophores/physiology
  • Mutation/genetics
  • Neural Crest/physiology
  • Phenotype
  • Pigmentation/genetics
  • Transcriptome/genetics
  • Zebrafish/genetics*
  • Zebrafish/physiology*
PubMed
31138706 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Understanding genetic and cellular bases of adult form remains a fundamental goal at the intersection of developmental and evolutionary biology. The skin pigment cells of vertebrates, derived from embryonic neural crest, are a useful system for elucidating mechanisms of fate specification, pattern formation, and how particular phenotypes impact organismal behavior and ecology. In a survey of Danio fishes, including the zebrafish Danio rerio, we identified two populations of white pigment cells-leucophores-one of which arises by transdifferentiation of adult melanophores and another of which develops from a yellow-orange xanthophore or xanthophore-like progenitor. Single-cell transcriptomic, mutational, chemical, and ultrastructural analyses of zebrafish leucophores revealed cell-type-specific chemical compositions, organelle configurations, and genetic requirements. At the organismal level, we identified distinct physiological responses of leucophores during environmental background matching, and we showed that leucophore complement influences behavior. Together, our studies reveal independently arisen pigment cell types and mechanisms of fate acquisition in zebrafish and illustrate how concerted analyses across hierarchical levels can provide insights into phenotypes and their evolution.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping