PUBLICATION
Prostaglandin signaling regulates renal multiciliated cell specification and maturation
- Authors
- Marra, A.N., Adeeb, B.D., Chambers, B.E., Drummond, B.E., Ulrich, M., Addiego, A., Springer, M., Poureetezadi, S.J., Chambers, J.M., Ronshaugen, M., Wingert, R.A.
- ID
- ZDB-PUB-190406-9
- Date
- 2019
- Source
- Proceedings of the National Academy of Sciences of the United States of America 116(17): 8409-8418 (Journal)
- Registered Authors
- Chambers, Brooke, Chambers, Joseph, Drummond, Bridgette, Marra, Amanda, Poureetezadi, Shahram, Springer, Meghan, Ulrich, Marisa, Wingert, Rebecca
- Keywords
- chemical screen, kidney, pronephros, prostaglandin, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation*/genetics
- Cell Differentiation*/physiology
- Cilia/metabolism*
- Epithelial Cells/cytology
- Epithelial Cells/physiology
- Epithelium/metabolism
- Epithelium/physiology
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology
- Kidney*/cytology
- Kidney*/growth & development
- Kidney*/metabolism
- Prostaglandins*/genetics
- Prostaglandins*/metabolism
- Signal Transduction*/genetics
- Signal Transduction*/physiology
- Zebrafish
- PubMed
- 30948642 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Marra, A.N., Adeeb, B.D., Chambers, B.E., Drummond, B.E., Ulrich, M., Addiego, A., Springer, M., Poureetezadi, S.J., Chambers, J.M., Ronshaugen, M., Wingert, R.A. (2019) Prostaglandin signaling regulates renal multiciliated cell specification and maturation. Proceedings of the National Academy of Sciences of the United States of America. 116(17):8409-8418.
Abstract
Multiciliated cells (MCCs) are specialized epithelia with apical bundles of motile cilia that direct fluid flow. MCC dysfunction is associated with human diseases of the respiratory, reproductive, and central nervous systems. Further, the appearance of renal MCCs has been cataloged in several kidney conditions, where their function is unknown. Despite their pivotal health importance, many aspects of MCC development remain poorly understood. Here, we utilized a chemical screen to identify molecules that affect MCC ontogeny in the zebrafish embryo kidney, and found prostaglandin signaling is essential both for renal MCC progenitor formation and terminal differentiation. Moreover, we show that prostaglandin activity is required downstream of the transcription factor ets variant 5a (etv5a) during MCC fate choice, where modulating prostaglandin E2 (PGE2) levels rescued MCC number. The discovery that prostaglandin signaling mediates renal MCC development has broad implications for other tissues, and could provide insight into a multitude of pathological states.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping