PUBLICATION
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis
- Authors
- Tu, H.Q., Qin, X.H., Liu, Z.B., Song, Z.Q., Hu, H.B., Zhang, Y.C., Chang, Y., Wu, M., Huang, Y., Bai, Y.F., Wang, G., Han, Q.Y., Li, A.L., Zhou, T., Liu, F., Zhang, X.M., Li, H.Y.
- ID
- ZDB-PUB-190131-1
- Date
- 2018
- Source
- Nature communications 9: 5277 (Journal)
- Registered Authors
- Liu, Feng
- Keywords
- none
- MeSH Terms
-
- Animals
- Axoneme/genetics
- Axoneme/metabolism*
- Centrosome/metabolism
- Cilia/genetics
- Cilia/metabolism*
- Humans
- Microtubules/metabolism*
- Mitosis
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Spindle Apparatus/genetics
- Spindle Apparatus/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 30538248 Full text @ Nat. Commun.
Citation
Tu, H.Q., Qin, X.H., Liu, Z.B., Song, Z.Q., Hu, H.B., Zhang, Y.C., Chang, Y., Wu, M., Huang, Y., Bai, Y.F., Wang, G., Han, Q.Y., Li, A.L., Zhou, T., Liu, F., Zhang, X.M., Li, H.Y. (2018) Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Nature communications. 9:5277.
Abstract
Defective ciliogenesis causes human developmental diseases termed ciliopathies. Microtubule (MT) asters originating from centrosomes in mitosis ensure the fidelity of cell division by positioning the spindle apparatus. However, the function of microtubule asters in interphase remains largely unknown. Here, we reveal an essential role of MT asters in transition zone (TZ) assembly during ciliogenesis. We demonstrate that the centrosome protein FSD1, whose biological function is largely unknown, anchors MT asters to interphase centrosomes by binding to microtubules. FSD1 knockdown causes defective ciliogenesis and affects embryonic development in vertebrates. We further show that disruption of MT aster anchorage by depleting FSD1 or other known anchoring proteins delocalizes the TZ assembly factor Cep290 from centriolar satellites, and causes TZ assembly defects. Thus, our study establishes FSD1 as a MT aster anchorage protein and reveals an important function of MT asters anchored by FSD1 in TZ assembly during ciliogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping