PUBLICATION
Resveratrol-Induced Xenophagy Promotes Intracellular Bacteria Clearance in Intestinal Epithelial Cells and Macrophages
- Authors
- Al Azzaz, J., Rieu, A., Aires, V., Delmas, D., Chluba, J., Winckler, P., Bringer, M.A., Lamarche, J., Vervandier-Fasseur, D., Dalle, F., Lapaquette, P., Guzzo, J.
- ID
- ZDB-PUB-190130-9
- Date
- 2019
- Source
- Frontiers in immunology 9: 3149 (Journal)
- Registered Authors
- Keywords
- AIEC, autophagy, resveratrol, salmonella, xenophagy
- MeSH Terms
-
- Animals
- Autophagy/drug effects*
- Autophagy/immunology*
- Cell Line
- Enterocolitis/etiology
- Enterocolitis/metabolism
- Epithelial Cells/drug effects*
- Epithelial Cells/immunology*
- Epithelial Cells/microbiology
- Escherichia coli/immunology
- Host-Pathogen Interactions/drug effects
- Host-Pathogen Interactions/immunology
- Humans
- Intestinal Mucosa/drug effects
- Intestinal Mucosa/immunology
- Intestinal Mucosa/metabolism
- Intestinal Mucosa/microbiology
- Macrophages/drug effects*
- Macrophages/immunology*
- Macrophages/microbiology
- Mice
- Resveratrol/pharmacology*
- Salmonella typhimurium/immunology
- Zebrafish
- PubMed
- 30693000 Full text @ Front Immunol
Citation
Al Azzaz, J., Rieu, A., Aires, V., Delmas, D., Chluba, J., Winckler, P., Bringer, M.A., Lamarche, J., Vervandier-Fasseur, D., Dalle, F., Lapaquette, P., Guzzo, J. (2019) Resveratrol-Induced Xenophagy Promotes Intracellular Bacteria Clearance in Intestinal Epithelial Cells and Macrophages. Frontiers in immunology. 9:3149.
Abstract
Autophagy is a lysosomal degradation process that contributes to host immunity by eliminating invasive pathogens and the modulating inflammatory response. Several infectious and immune disorders are associated with autophagy defects, suggesting that stimulation of autophagy in these diseases should be beneficial. Here, we show that resveratrol is able to boost xenophagy, a selective form of autophagy that target invasive bacteria. We demonstrated that resveratrol promotes in vitro autophagy-dependent clearance of intracellular bacteria in intestinal epithelial cells and macrophages. These results were validated in vivo using infection in a transgenic GFP-LC3 zebrafish model. We also compared the ability of resveratrol derivatives, designed to improve the bioavailability of the parent molecule, to stimulate autophagy and to induce intracellular bacteria clearance. Together, our data demonstrate the ability of resveratrol to stimulate xenophagy, and thereby enhance the clearance of two invasive bacteria involved life-threatening diseases, Salmonella Typhimurium and Crohn's disease-associated Adherent-Invasive Escherichia coli. These findings encourage the further development of pro-autophagic nutrients to strengthen intestinal homeostasis in basal and infectious states.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping