PUBLICATION
Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline
- Authors
- Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., Sun, Y.
- ID
- ZDB-PUB-190124-3
- Date
- 2019
- Source
- Marine biotechnology (New York, N.Y.) 21(2): 217-228 (Journal)
- Registered Authors
- He, Mudan, Sun, Yonghua, Xiong, Feng, Ye, Ding, Zhu, Zuoyan
- Keywords
- Oogenesis, Oogonia, Primordial germ cell, Spermatogenesis, Spermatogonia, piwil1
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/embryology
- Embryonic Development*
- Female
- Gene Expression
- Germ Cells/cytology
- Luminescent Proteins
- Male
- Ovary/growth & development
- Sex Differentiation*
- Testis/growth & development
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins
- PubMed
- 30671659 Full text @ Mar. Biotechnol.
Citation
Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., Sun, Y. (2019) Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Marine biotechnology (New York, N.Y.). 21(2):217-228.
Abstract
Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe the generation and characterization of a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)ihb327Tg, which specifically labels the whole lifetime of germ cells, i.e., from primordial germ cells (PGCs) at shield stage to the oogonia and early stage of oocytes in the ovary and to the early stage of spermatogonia, spermatocyte, and spermatid in the testis. By using this transgenic line, we carefully observed the numbers of PGCs from early embryonic stage to juvenile stage and the differentiation process of ovary and testis. The numbers of PGCs became variable at as early as 1 day post-fertilization (dpf). Interestingly, the embryos with a high amount of PGCs mainly developed into females and the ones with a low amount of PGCs mainly developed into males. By using transient overexpression and transgenic induction of PGC-specific bucky ball (buc), we further proved that induction of abundant PGCs at embryonic stage promoted later ovary differentiation and female development. Taken together, we generate an ideal transgenic line Tg(piwil1:egfp-UTRnanos3)ihb327Tg which can visualize zebrafish germline for a lifetime, and we have utilized this line to study germ cell development and gonad differentiation of teleost and to demonstrate that the increase of PGC number at embryonic stage promotes female differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping