PUBLICATION
Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish
- Authors
- Saju, J.M., Hossain, M.S., Liew, W.C., Pradhan, A., Thevasagayam, N.M., Tan, L.S.E., Anand, A., Olsson, P.E., Orbán, L.
- ID
- ZDB-PUB-181220-13
- Date
- 2018
- Source
- Cell Reports 25: 3252-3261.e4 (Journal)
- Registered Authors
- Liew, Woei Chang, Pradhan, Ajay, Saju, Jolly M.
- Keywords
- CRISPR/Cas9, Hsf5, fish sex, gonad differentiation, meiosis, reproduction, sexual development
- MeSH Terms
-
- Animals
- Apoptosis
- Female
- Fertility
- Male
- Meiosis
- Mutation/genetics
- Sex Characteristics
- Spermatocytes/cytology
- Spermatocytes/metabolism
- Spermatocytes/ultrastructure
- Spermatogenesis*
- Spermatozoa/cytology
- Spermatozoa/ultrastructure
- Testis/cytology
- Testis/ultrastructure
- Transcriptome/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/metabolism*
- PubMed
- 30566854 Full text @ Cell Rep.
Citation
Saju, J.M., Hossain, M.S., Liew, W.C., Pradhan, A., Thevasagayam, N.M., Tan, L.S.E., Anand, A., Olsson, P.E., Orbán, L. (2018) Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish. Cell Reports. 25:3252-3261.e4.
Abstract
Heat shock factors (Hsfs) are transcription factors that regulate responses to heat shock and other environmental stimuli. Four heat shock factors (Hsf1-4) have been characterized from vertebrates to date. In addition to stress response, they also play important roles in development and gametogenesis. Here, we study the fifth member of heat shock factor family, Hsf5, using zebrafish as a model organism. Mutant hsf5-/- males, generated by CRISPR/Cas9 technique, were infertile with drastically reduced sperm count, increased sperm head size, and abnormal tail architecture, whereas females remained fertile. We show that Hsf5 is required for progression through meiotic prophase 1 during spermatogenesis as suggested by the accumulation of cells in the leptotene and zygotene-pachytene stages and increased apoptosis in post-meiotic cells. hsf5-/- mutants show gonadal misregulation of a substantial number of genes with roles in cell cycle, apoptosis, protein modifications, and signal transduction, indicating an important role of Hsf5 in early stages of spermatogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping