PUBLICATION
The Aryl Hydrocarbon Receptor Pathway Defines the Time Frame for Restorative Neurogenesis
- Authors
- Di Giaimo, R., Durovic, T., Barquin, P., Kociaj, A., Lepko, T., Aschenbroich, S., Breunig, C.T., Irmler, M., Cernilogar, F.M., Schotta, G., Barbosa, J.S., Trümbach, D., Baumgart, E.V., Neuner, A.M., Beckers, J., Wurst, W., Stricker, S.H., Ninkovic, J.
- ID
- ZDB-PUB-181220-12
- Date
- 2018
- Source
- Cell Reports 25: 3241-3251.e5 (Journal)
- Registered Authors
- Beckers, Johannes, Ninkovic, Jovica
- Keywords
- aryl hydrocarbon receptor, direct conversion, live imaging, neurogenesis, regeneration, zebrafish
- Datasets
- GEO:GSE121404, GEO:GSE102400
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Proliferation
- Cell Survival
- Ependymoglial Cells/cytology
- Ependymoglial Cells/metabolism
- Mitosis
- Neurogenesis*
- Neurons/cytology
- Receptors, Aryl Hydrocarbon/metabolism*
- Signal Transduction*
- Time Factors
- Zebrafish
- PubMed
- 30566853 Full text @ Cell Rep.
Citation
Di Giaimo, R., Durovic, T., Barquin, P., Kociaj, A., Lepko, T., Aschenbroich, S., Breunig, C.T., Irmler, M., Cernilogar, F.M., Schotta, G., Barbosa, J.S., Trümbach, D., Baumgart, E.V., Neuner, A.M., Beckers, J., Wurst, W., Stricker, S.H., Ninkovic, J. (2018) The Aryl Hydrocarbon Receptor Pathway Defines the Time Frame for Restorative Neurogenesis. Cell Reports. 25:3241-3251.e5.
Abstract
Zebrafish have a high capacity to replace lost neurons after brain injury. New neurons involved in repair are generated by a specific set of glial cells, known as ependymoglial cells. We analyze changes in the transcriptome of ependymoglial cells and their progeny after injury to infer the molecular pathways governing restorative neurogenesis. We identify the aryl hydrocarbon receptor (AhR) as a regulator of ependymoglia differentiation toward post-mitotic neurons. In vivo imaging shows that high AhR signaling promotes the direct conversion of a specific subset of ependymoglia into post-mitotic neurons, while low AhR signaling promotes ependymoglial proliferation. Interestingly, we observe the inactivation of AhR signaling shortly after injury followed by a return to the basal levels 7 days post injury. Interference with timely AhR regulation after injury leads to aberrant restorative neurogenesis. Taken together, we identify AhR signaling as a crucial regulator of restorative neurogenesis timing in the zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping