PUBLICATION
Identification of novel circadian transcripts in the zebrafish retina
- Authors
- Ramasamy, S., Sharma, S., Iyengar, B.R., Vellarikkal, S.K., Sivasubbu, S., Maiti, S., Pillai, B.
- ID
- ZDB-PUB-181118-16
- Date
- 2018
- Source
- The Journal of experimental biology 222(Pt 1): (Journal)
- Registered Authors
- Pillai, Beena, Sivasubbu, Sridhar
- Keywords
- Circadian rhythms, RNA-seq, Retina, Transcriptomics, Zebrafish
- MeSH Terms
-
- Animals
- Circadian Rhythm/physiology*
- Retina/physiology
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30446534 Full text @ J. Exp. Biol.
Citation
Ramasamy, S., Sharma, S., Iyengar, B.R., Vellarikkal, S.K., Sivasubbu, S., Maiti, S., Pillai, B. (2018) Identification of novel circadian transcripts in the zebrafish retina. The Journal of experimental biology. 222(Pt 1):.
Abstract
High fecundity, transparent embryos for monitoring the rapid development of organs and the availability of a well-annotated genome has made zebrafish a model organism of choice for developmental biology and neurobiology. This vertebrate model, a favourite in chronobiology studies, shows striking circadian rhythmicity in behaviour. Here, we identify novel genes in the zebrafish genome, which are expressed in the zebrafish retina. We further resolve the expression pattern over time and tentatively assign specific novel transcripts to retinal bipolar cells of the inner nuclear layer. Using chemical ablation and free run experiments we segregate the transcripts that are rhythmic when entrained by light from those that show sustained oscillations in the absence of external cues. The transcripts reported here with rigorous annotation and specific functions in circadian biology provide the groundwork for functional characterisation of novel players in the zebrafish retinal clock.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping