PUBLICATION

Prolactin controls branchial clcn2c but not atp1a1a.2 in zebrafish Danio rerio

Authors
Breves, J.P.
ID
ZDB-PUB-181028-7
Date
2018
Source
Journal of Fish Biology   94(1): 168-172 (Journal)
Registered Authors
Keywords
Cl- channel, Na+-K+-ATPase, gill, ionocyte, prolactin, zebrafish
MeSH Terms
  • Animals
  • Chloride Channels/genetics
  • Chloride Channels/metabolism*
  • Chlorine/metabolism
  • Fresh Water/chemistry
  • Gene Expression Regulation/drug effects
  • Gills/metabolism
  • Homeostasis
  • Prolactin/pharmacology*
  • Protein Transport
  • RNA, Messenger/metabolism
  • Sodium Chloride Symporters/metabolism
  • Sodium-Potassium-Exchanging ATPase/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
30367725 Full text @ J. Fish Biol.
Abstract
This study examined the branchial epithelium of stenohaline zebrafish Danio rerio, Na+ -Cl- cotransporter-like 2 (Slc12a10.2)-expressing ionocytes [Na+ -Cl- cotransporter (Ncc)-cells], which mediate the active uptake of ions from freshwater environments. The study assessed whether the pituitary hormone prolactin (Prl) stimulates the expression of messenger (m)RNAs encoding a Clc Cl- channel family member (clcn2c) and a Na+ -K+ -ATPase α1 subunit (atp1a1a.2) expressed in Ncc-cells. Branchial clcn2c, but not atp1a1a.2 levels, were sensitive to Prl both in vitro and in vivo. These observations suggest that Prl contributes to maintaining systemic Cl- balance via the regulation of clcn2c. This article is protected by copyright. All rights reserved.
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