PUBLICATION
The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish
- Authors
- Dingare, C., Niedzwetzki, A., Klemmt, P.A., Godbersen, S., Fuentes, R., Mullins, M.C., Lecaudey, V.
- ID
- ZDB-PUB-181018-2
- Date
- 2018
- Source
- Development (Cambridge, England) 145(22): (Journal)
- Registered Authors
- Lecaudey, Virginie, Mullins, Mary C.
- Keywords
- Fertilization, Hippo, Micropylar cell, Micropyle, Taz, Zebrafish
- MeSH Terms
-
- Adherens Junctions/drug effects
- Adherens Junctions/metabolism
- Animals
- Biomarkers/metabolism
- Cell Polarity/drug effects
- Cell Shape/drug effects
- Cytochalasin D/pharmacology
- Female
- Fertilization*/drug effects
- Infertility, Female/genetics
- Infertility, Female/pathology
- Intracellular Signaling Peptides and Proteins/metabolism*
- Microtubules/drug effects
- Microtubules/metabolism
- Models, Biological
- Morphogenesis*/drug effects
- Mutation/genetics
- Oocytes/drug effects
- Oocytes/metabolism
- Oocytes/pathology
- Ovum/drug effects
- Ovum/metabolism
- Protein Serine-Threonine Kinases/metabolism*
- Signal Transduction*
- Tight Junctions/drug effects
- Tight Junctions/metabolism
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- PubMed
- 30327325 Full text @ Development
Citation
Dingare, C., Niedzwetzki, A., Klemmt, P.A., Godbersen, S., Fuentes, R., Mullins, M.C., Lecaudey, V. (2018) The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish. Development (Cambridge, England). 145(22):.
Abstract
Hippo signaling is a critical pathway integrating extrinsic and intrinsic mechanical cues to regulate organ size. Despite its essential role in organogenesis, little is known about its role in cell fate specification and differentiation. Here we unravel a novel and unexpected role of the Hippo pathway effector Taz (wwtr1) in controlling the size, shape and fate of a unique cell in the zebrafish ovary. We show that wwtr1 mutant females are infertile. In teleosts, fertilization occurs through the micropyle, a funnel-like opening in the chorion, formed by a unique, enlarged follicle cell, the micropylar cell (MC). We describe here for the first time the mechanism underlying the differentiation of the MC. Our genetic analyses show that Taz is essential for MC fate acquisition and subsequent micropyle formation in zebrafish. We identify Taz as the first bona fide MC marker and show that Taz is specifically and strongly enriched in the MC precursor. Altogether, we performed the first genetic and molecular characterization of the MC and propose that Taz is a key regulator of the MC fate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping