PUBLICATION
Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response
- Authors
- Chen, L., Yan, J., Shi, J., Sun, W., Chen, Z., Yu, J., Qi, J., Du, Y., Zhang, H., Feng, L.
- ID
- ZDB-PUB-180910-4
- Date
- 2018
- Source
- Fish & shellfish immunology 83: 96-103 (Journal)
- Registered Authors
- Keywords
- Bacterial carbohydrates, Carbohydrate recognition domain, Innate immunity, Intelectin, Zebrafish
- MeSH Terms
-
- Agglutination
- Animals
- Cytokines/genetics
- Cytokines/immunology*
- Escherichia coli
- Fish Diseases/immunology*
- Gram-Negative Bacteria
- Gram-Positive Bacteria
- Immunity, Innate*
- Lectins/genetics
- Lectins/immunology*
- Lipopolysaccharides
- Peptidoglycan/metabolism
- Phylogeny
- Recombinant Proteins/genetics
- Recombinant Proteins/immunology
- Staphylococcus aureus
- Zebrafish/immunology*
- PubMed
- 30195915 Full text @ Fish Shellfish Immunol.
Citation
Chen, L., Yan, J., Shi, J., Sun, W., Chen, Z., Yu, J., Qi, J., Du, Y., Zhang, H., Feng, L. (2018) Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response. Fish & shellfish immunology. 83:96-103.
Abstract
Intelectin displays carbohydrate binding capacity and has been demonstrated to agglutinate bacteria, suggesting its role in innate immunity. It has also been linked to many pathogenic conditions in human. After reporting two amphioxus orthologs and the zebrafish intelectin 2 (zITLN2), here we cloned and characterized zebrafish intelectin 1 (zITLN1). Like zITLN2, zITLN1 also contains a conserved fibrinogen-related domain (FReD) and a unique intelectin domain (ITLN-D), expresses in all the tissues tested, with the highest level in intestine, and responds to bacterial challenge in acute phase. We also expressed zITLN1 in E. coli system, and purified recombinant zITLN1 could agglutinate both Gram-positive and Gram-negative bacteria in a calcium dependent manner. Its ability to agglutinate Gram-positive bacteria is stronger than that to Gram-negative bacteria whereas zITLN2 did not show such preference. This is probably due to the fact that recombinant zITLN1 could bind peptidoglycan (PGN) with a higher degree to lipopolysaccharide (LPS). Our results of zITLN1 provided new insight into the evolution and function of the intelectin family.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping