PUBLICATION
Myricanol mitigates lipid accumulation in 3T3-L1 adipocytes and high fat diet-fed zebrafish via activating AMP-activated protein kinase
- Authors
- Shen, S., Liao, Q., Feng, Y., Liu, J., Pan, R., Lee, S.M., Lin, L.
- ID
- ZDB-PUB-180904-1
- Date
- 2019
- Source
- Food Chemistry 270: 305-314 (Journal)
- Registered Authors
- Keywords
- AMP-activated protein kinase, Adipogenesis, Insulin sensitivity, Lipid accumulation, Myricanol, Zebrafish
- MeSH Terms
-
- 3T3-L1 Cells
- AMP-Activated Protein Kinases/metabolism*
- Adipocytes/cytology
- Adipocytes/drug effects
- Adipogenesis*/drug effects
- Animals
- CCAAT-Enhancer-Binding Protein-alpha
- Diarylheptanoids/pharmacology*
- Diet, High-Fat/adverse effects
- Lipids
- Mice
- PPAR gamma
- Zebrafish*
- PubMed
- 30174051 Full text @ Food Chem.
Citation
Shen, S., Liao, Q., Feng, Y., Liu, J., Pan, R., Lee, S.M., Lin, L. (2019) Myricanol mitigates lipid accumulation in 3T3-L1 adipocytes and high fat diet-fed zebrafish via activating AMP-activated protein kinase. Food Chemistry. 270:305-314.
Abstract
Myricanol is a diarylheptanoid isolated from Chinese bayberry. Through virtual docking strategy, myricanol was discovered as an AMP-activated protein kinase (AMPK) activator among a series of structural analogs, with high affinity for the γ subunit of AMPK. Myricanol was also evaluated for regulatory effects on lipid accumulation and insulin sensitivity in 3T3-L1 adipocytes and adiposity in high-fat diet-fed zebrafish. Myricanol suppressed lipid accumulation in 3T3-L1 cells in the initial stage (days 0-2) by suppressing adipogenesis and in the terminal stage (days 4-7) by inducing lipolysis and lipid combustion through activating AMPK. Moreover, myricanol enhanced insulin-stimulated glucose uptake by activating the insulin signaling pathway. In high-fat diet-fed zebrafish, myricanol inhibited lipid accumulation by suppressing adipogenic factors including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). In summary, the results indicate that myricanol could be a potential therapeutic agent against obesity by activating the AMPK signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping