PUBLICATION
Neutrophils use superoxide to control bacterial infection at a distance
- Authors
- Phan, Q.T., Sipka, T., Gonzalez, C., Levraud, J.P., Lutfalla, G., Nguyen-Chi, M.
- ID
- ZDB-PUB-180718-9
- Date
- 2018
- Source
- PLoS pathogens 14: e1007157 (Journal)
- Registered Authors
- Levraud, Jean-Pierre, Lutfalla, Georges, Phan, Quang Tien
- Keywords
- none
- MeSH Terms
-
- Animals
- Escherichia coli/immunology
- Escherichia coli Infections/immunology*
- Neutrophils/immunology*
- Superoxides/immunology*
- Zebrafish
- PubMed
- 30016370 Full text @ PLoS Pathog.
Citation
Phan, Q.T., Sipka, T., Gonzalez, C., Levraud, J.P., Lutfalla, G., Nguyen-Chi, M. (2018) Neutrophils use superoxide to control bacterial infection at a distance. PLoS pathogens. 14:e1007157.
Abstract
Understanding the roles of neutrophils and macrophages in fighting bacterial infections is a critical issue in human pathologies. Although phagocytic killing has been extensively studied, little is known about how bacteria are eliminated extracellularly in live vertebrates. We have recently developed an infection model in the zebrafish embryo in which leukocytes cannot reach the injected bacteria. When Escherichia coli bacteria are injected within the notochord, both neutrophils and macrophages are massively recruited during several days, but do not infiltrate the infected tissue presumably because of its tough collagen sheath. Nevertheless, the bacteria are killed during the first 24 hours, and we report here that neutrophils, but not macrophages are involved in the control of the infection. Using genetic and chemical approaches, we show that even in absence of phagocytosis, the bactericidal action relies on NADPH oxidase-dependent production of superoxide in neutrophils. We thus reveal a host effector mechanism mediated by neutrophils that eliminates bacteria that cannot be reached by phagocytes and that is independent of macrophages, NO synthase or myeloperoxidase.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping