PUBLICATION
Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly
- Authors
- Yamaguchi, H., Oda, T., Kikkawa, M., Takeda, H.
- ID
- ZDB-PUB-180510-7
- Date
- 2018
- Source
- eLIFE 7: (Journal)
- Registered Authors
- Takeda, Hiroyuki
- Keywords
- cell biology, molecular biophysics, structural biology, zebrafish
- MeSH Terms
-
- Animals
- Axonemal Dyneins/metabolism*
- Axonemal Dyneins/ultrastructure
- Cell Movement
- Cilia/chemistry
- Cilia/physiology
- Cryoelectron Microscopy
- Flagella/chemistry
- Flagella/physiology
- Male
- Motion
- Protein Multimerization*
- Spermatozoa/chemistry*
- Spermatozoa/physiology*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29741156 Full text @ Elife
Citation
Yamaguchi, H., Oda, T., Kikkawa, M., Takeda, H. (2018) Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly. eLIFE. 7.
Abstract
Construction of motile cilia/flagella requires cytoplasmic preassembly of axonemal dyneins before transport into cilia. Axonemal dyneins have various subtypes, but the roles of each dynein subtype and their assembly processes remain elusive in vertebrates. The PIH protein family, consisting of four members, has been implicated in the assembly of different dynein subtypes, although evidence for this idea is sparse. Here, we established zebrafish mutants of all four PIH-protein genes: pih1d1, pih1d2, ktu, and twister, and analyzed the structures of axonemal dyneins in mutant spermatozoa by cryo-electron tomography. Mutations caused the loss of specific dynein subtypes, which was correlated with abnormal sperm motility. We also found organ-specific compositions of dynein subtypes, which could explain the severe motility defects of mutant Kupffer's vesicle cilia. Our data demonstrate that all vertebrate PIH proteins are differently required for cilia/flagella motions and the assembly of axonemal dyneins, assigning specific dynein subtypes to each PIH protein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping