PUBLICATION
Chlorella diet alters mitochondrial cardiolipin contents differentially in organs of Danio rerio analyzed by a lipidomics approach
- Authors
- Chao, Y.J., Wu, W.H., Balazova, M., Wu, T.Y., Lin, J., Liu, Y.W., Hsu, Y.H.
- ID
- ZDB-PUB-180302-6
- Date
- 2018
- Source
- PLoS One 13: e0193042 (Journal)
- Registered Authors
- Liu, Yi-wen
- Keywords
- none
- MeSH Terms
-
- Animal Feed/analysis
- Animal Nutritional Physiological Phenomena
- Animals
- Barth Syndrome/metabolism
- Cardiolipins/analysis
- Cardiolipins/metabolism*
- Chlorella/metabolism
- Diet*
- Energy Metabolism
- Female
- Mitochondria/metabolism*
- Zebrafish/physiology*
- PubMed
- 29494608 Full text @ PLoS One
Citation
Chao, Y.J., Wu, W.H., Balazova, M., Wu, T.Y., Lin, J., Liu, Y.W., Hsu, Y.H. (2018) Chlorella diet alters mitochondrial cardiolipin contents differentially in organs of Danio rerio analyzed by a lipidomics approach. PLoS One. 13:e0193042.
Abstract
The zebrafish (Danio rerio) is an important and widely used vertebrate model organism for the study of human diseases which include disorders caused by dysfunctional mitochondria. Mitochondria play an essential role in both energy metabolism and apoptosis, which are mediated through a mitochondrial phospholipid cardiolipin (CL). In order to examine the cardiolipin profile in the zebrafish model, we developed a CL analysis platform by using liquid chromatography-mass spectrometry (LC-MS). Meanwhile, we tested whether chlorella diet would alter the CL profile in the larval fish, and in various organs of the adult fish. The results showed that chlorella diet increased the chain length of CL in larval fish. In the adult zebrafish, the distribution patterns of CL species were similar between the adult brain and eye tissues, and between the heart and muscles. Interestingly, monolyso-cardiolipin (MLCL) was not detected in brain and eyes but found in other examined tissues, indicating a different remodeling mechanism to maintain the CL integrity. While the adult zebrafish were fed with chlorella for four weeks, the CL distribution showed an increase of the species of saturated acyl chains in the brain and eyes, but a decrease in the other organs. Moreover, chlorella diet led to a decrease of MLCL percentage in organs except the non-MLCL-containing brain and eyes. The CL analysis in the zebrafish provides an important tool for studying the mechanism of mitochondria diseases, and may also be useful for testing medical regimens targeting against the Barth Syndrome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping