PUBLICATION
Transcripts within rod photoreceptors of the Zebrafish retina
- Authors
- Sun, C., Galicia, C., Stenkamp, D.L.
- ID
- ZDB-PUB-180210-2
- Date
- 2018
- Source
- BMC Genomics 19: 127 (Journal)
- Registered Authors
- Stenkamp, Deborah L.
- Keywords
- RNA-Seq, Regeneration, Retina, Rod photoreceptor, Transcriptome, Zebrafish
- Datasets
- GEO:GSE100062
- MeSH Terms
-
- Animals
- High-Throughput Nucleotide Sequencing
- Zebrafish/genetics*
- Retinal Degeneration/genetics
- Retinal Degeneration/metabolism
- Retinal Degeneration/pathology
- Retina/cytology*
- Retina/metabolism
- Retina/pathology
- Fluorescent Antibody Technique
- Gene Expression Profiling
- Gene Ontology
- Gene Library
- Retinal Rod Photoreceptor Cells/metabolism*
- Regeneration
- Molecular Sequence Annotation
- Computational Biology/methods
- Transcriptome*
- PubMed
- 29422031 Full text @ BMC Genomics
Citation
Sun, C., Galicia, C., Stenkamp, D.L. (2018) Transcripts within rod photoreceptors of the Zebrafish retina. BMC Genomics. 19:127.
Abstract
Background The purpose of this study was to identify transcripts of retinal rod photoreceptors of the zebrafish. The zebrafish is an important animal model for vision science due to rapid and tractable development, persistent neurogenesis of rods throughout the lifespan, and capacity for functional retinal regeneration.
Results Zebrafish rods, and non-rod retinal cells of the xops:eGFP transgenic line, were separated by cell dissociation and fluorescence-activated cell sorting (FACS), followed by RNA-seq. At a false discovery rate of
Conclusions We report the generation and validation of an RNA-seq dataset describing the rod transcriptome of the zebrafish, which is now available as a resource for further studies of rod photoreceptor biology and comparative transcriptomics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping