PUBLICATION
Ovulation is associated with the LH-dependent induction of pla2g4aa in zebrafish
- Authors
- Tang, H., Wang, L., Chen, Y., He, J., Qu, L., Guo, Y., Liu, Y., Liu, X., Lin, H.
- ID
- ZDB-PUB-180113-12
- Date
- 2018
- Source
- Molecular and Cellular Endocrinology 473: 53-60 (Journal)
- Registered Authors
- Liu, Xiaochun
- Keywords
- LH, Ovulation, PG, Zebrafish, pla2g4aa
- MeSH Terms
-
- Animals
- Arachidonic Acids/pharmacology
- Cells, Cultured
- Chorionic Gonadotropin/pharmacology
- Down-Regulation/drug effects
- Female
- Gene Expression Regulation/drug effects
- Humans
- Luteinizing Hormone/pharmacology*
- Mutation/genetics
- Ovarian Follicle/drug effects
- Ovarian Follicle/metabolism
- Ovulation/drug effects*
- Phospholipase A2 Inhibitors/pharmacology
- Phospholipases A2/metabolism
- Prostaglandins/metabolism
- Signal Transduction/drug effects
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29326060 Full text @ Mol. Cell. Endocrinol.
Citation
Tang, H., Wang, L., Chen, Y., He, J., Qu, L., Guo, Y., Liu, Y., Liu, X., Lin, H. (2018) Ovulation is associated with the LH-dependent induction of pla2g4aa in zebrafish. Molecular and Cellular Endocrinology. 473:53-60.
Abstract
The effects of the preovulatory luteinizing hormone (LH) surge on the ovulatory process are mediated by prostaglandins (PGs), the synthesis of which involves prostaglandin synthetase and cytosolic phospholipase A2 (cPLA2). In our previous study, we systematically investigated the function of prostaglandin endoperoxide synthase (ptgs) genes on ovulation in zebrafish. However, the role of cPLA2 in ovulation was not determined in zebrafish. In this study, we investigated the function of cpla2α in PGs production and ovulation in periovulatory follicles. Our data showed that the expression of pla2g4aa increased during zebrafish folliculogenesis and the follicular layer was the primary region with expression of pla2g4aa. In addition, the expression of pla2g4aa was regulated by LH in vitro and in vivo. Furthermore, injection of AACOCF3, a specific inhibitor of cPLA2, significantly reduced ovarian PGs level and blocked hCG-induced ovulation. Collectively, these findings suggest that pla2g4aa is related to the ovulation process in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping