PUBLICATION
Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry
- Authors
- Ery?lmaz, O., Ate?, P.S., Ünal, ?., Üstünda?, Ü.V., Bay, S., Alturfan, A.A., Yi?itba??, T., Emekli-Alturfan, E., Akal?n, M.
- ID
- ZDB-PUB-171229-3
- Date
- 2017
- Source
- Journal of biochemical and molecular toxicology 32(1): (Journal)
- Registered Authors
- Ate?, Perihan Seda, Emekli-Alturfan, Ebru, Unal, Ismail, Üstünda?, Ünsal Veli
- Keywords
- Wnt pathway, apoptosis, oxidant-antioxidant status, silver nanoparticles, zebrafish embryo
- MeSH Terms
-
- Dose-Response Relationship, Drug
- Apoptosis/drug effects*
- Metal Nanoparticles/administration & dosage
- Metal Nanoparticles/toxicity*
- Embryonic Development/drug effects
- Gene Expression Regulation, Developmental/drug effects
- Lipid Peroxidation/drug effects
- Oxidative Stress/drug effects*
- Cyclin D1/antagonists & inhibitors
- Cyclin D1/genetics
- Cyclin D1/metabolism
- Silver/administration & dosage
- Silver/toxicity*
- Animals
- Water Pollutants, Chemical/administration & dosage
- Water Pollutants, Chemical/toxicity*
- Zebrafish Proteins/agonists
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Enzyme Induction/drug effects
- Glycogen Synthase Kinase 3 beta/chemistry
- Glycogen Synthase Kinase 3 beta/genetics
- Glycogen Synthase Kinase 3 beta/metabolism
- Teratogens/toxicity
- Wnt Signaling Pathway/drug effects*
- Zebrafish
- Disinfectants/toxicity
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Cell Proliferation/drug effects
- PubMed
- 29283201 Full text @ J. Biochem. Mol. Toxicol.
- CTD
- 29283201
Citation
Ery?lmaz, O., Ate?, P.S., Ünal, ?., Üstünda?, Ü.V., Bay, S., Alturfan, A.A., Yi?itba??, T., Emekli-Alturfan, E., Akal?n, M. (2017) Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry. Journal of biochemical and molecular toxicology. 32(1).
Abstract
Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Aim was to evaluate the effects of AgNPs on the development of zebrafish embryos focusing on Wnt pathway, proliferation, oxidant-antioxidant status, and apoptosis. The expressions of ccnd1 and gsk3? were determined by RT-PCR, whereas ?-catenin and proliferative cell antigen (PCNA) expressions were determined immunohistochemically. Lipid peroxidation, superoxide dismutase, and glutathione-S-transferase activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Oxidant status, apoptosis, immunohistochemical PCNA, and ? catenin staining increased, whereas ccnd1 and antioxidant enzyme activities decreased in AgNPs-exposed embryos in a dose-dependent manner. Our results indicate the interaction of possible mechanisms that may be responsible for the toxic effects of AgNPs in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping