PUBLICATION
WDR11-mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome
- Authors
- Kim, Y.J., Osborn, D.P., Lee, J.Y., Araki, M., Araki, K., Mohun, T., Känsäkoski, J., Brandstack, N., Kim, H.T., Miralles, F., Kim, C.H., Brown, N.A., Kim, H.G., Martinez-Barbera, J.P., Ataliotis, P., Raivio, T., Layman, L.C., Kim, S.H.
- ID
- ZDB-PUB-171222-2
- Date
- 2017
- Source
- EMBO reports 19(2): 269-289 (Journal)
- Registered Authors
- Kim, Cheol-Hee, Kim, Hyun-Taek
- Keywords
- WDR11, ciliopathy, hedgehog signal pathway, hypogonadotropic hypogonadism, kallmann syndrome
- MeSH Terms
-
- Animals
- Biopsy
- Ciliopathies/genetics*
- Ciliopathies/metabolism*
- Gene Expression
- Gene Expression Profiling
- Gene Knockout Techniques
- Genetic Association Studies
- Genotype
- Hedgehog Proteins/metabolism*
- Humans
- Kallmann Syndrome/diagnosis
- Kallmann Syndrome/genetics*
- Kallmann Syndrome/metabolism*
- Magnetic Resonance Imaging
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mice
- Mice, Knockout
- Mutation
- Organ Specificity/genetics
- Patched-1 Receptor/genetics
- Phenotype
- Promoter Regions, Genetic
- Protein Binding
- Protein Transport
- Signal Transduction*
- Transcriptome
- Zebrafish
- PubMed
- 29263200 Full text @ EMBO Rep.
Citation
Kim, Y.J., Osborn, D.P., Lee, J.Y., Araki, M., Araki, K., Mohun, T., Känsäkoski, J., Brandstack, N., Kim, H.T., Miralles, F., Kim, C.H., Brown, N.A., Kim, H.G., Martinez-Barbera, J.P., Ataliotis, P., Raivio, T., Layman, L.C., Kim, S.H. (2017) WDR11-mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome. EMBO reports. 19(2):269-289.
Abstract
WDR11 has been implicated in congenital hypogonadotropic hypogonadism (CHH) and Kallmann syndrome (KS), human developmental genetic disorders defined by delayed puberty and infertility. However, WDR11's role in development is poorly understood. Here, we report that WDR11 modulates the Hedgehog (Hh) signalling pathway and is essential for ciliogenesis. Disruption of WDR11 expression in mouse and zebrafish results in phenotypic characteristics associated with defective Hh signalling, accompanied by dysgenesis of ciliated tissues. Wdr11-null mice also exhibit early-onset obesity. We find that WDR11 shuttles from the cilium to the nucleus in response to Hh signalling. WDR11 regulates the proteolytic processing of GLI3 and cooperates with the transcription factor EMX1 in the induction of downstream Hh pathway gene expression and gonadotrophin-releasing hormone production. The CHH/KS-associated human mutations result in loss of function of WDR11. Treatment with the Hh agonist purmorphamine partially rescues the WDR11 haploinsufficiency phenotypes. Our study reveals a novel class of ciliopathy caused by WDR11 mutations and suggests that CHH/KS may be a part of the human ciliopathy spectrum.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping