PUBLICATION
Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance
- Authors
- Ando, K., Shibata, E., Hans, S., Brand, M., Kawakami, A.
- ID
- ZDB-PUB-171107-14
- Date
- 2017
- Source
- Developmental Cell 43(5): 643-650.e3 (Journal)
- Registered Authors
- Ando, Kazunori, Brand, Michael, Hans, Stefan, Kawakami, Atsushi
- Keywords
- bone regeneration, cell ablation, cre-loxP, fin, matrix metalloproteinase 9, osteoblast progenitor cells, stem cells, transplantation, zebrafish
- MeSH Terms
-
- Animals
- Bone Regeneration/physiology*
- Bone and Bones/cytology*
- Cell Dedifferentiation/physiology
- Cell Lineage/physiology
- Cell Proliferation/physiology*
- Osteoblasts/cytology*
- Regeneration/physiology*
- Stem Cells/cytology*
- Zebrafish*/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 29103952 Full text @ Dev. Cell
Citation
Ando, K., Shibata, E., Hans, S., Brand, M., Kawakami, A. (2017) Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance. Developmental Cell. 43(5):643-650.e3.
Abstract
Mammals cannot re-form heavily damaged bones as in large fracture gaps, whereas zebrafish efficiently regenerate bones even after amputation of appendages. However, the source of osteoblasts that mediate appendage regeneration is controversial. Several studies in zebrafish have shown that osteoblasts are generated by dedifferentiation of existing osteoblasts at injured sites, but other observations suggest that de novo production of osteoblasts also occurs. In this study, we found from cell-lineage tracing and ablation experiments that a group of cells reserved in niches serves as osteoblast progenitor cells (OPCs) and has a significant role in fin ray regeneration. Besides regeneration, OPCs also supply osteoblasts for normal bone maintenance. We further showed that OPCs are derived from embryonic somites, as is the case with embryonic osteoblasts, and are replenished from mesenchymal precursors in adult zebrafish. Our findings reveal that reserved progenitors are a significant and complementary source of osteoblasts for zebrafish bone regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping