PUBLICATION
Diminished PRRX1 Expression Is Associated With Increased Risk of Atrial Fibrillation and Shortening of the Cardiac Action Potential.
- Authors
- Tucker, N.R., Dolmatova, E.V., Lin, H., Cooper, R.R., Ye, J., Hucker, W.J., Jameson, H.S., Parsons, V.A., Weng, L.C., Mills, R.W., Sinner, M.F., Imakaev, M., Leyton-Mange, J., Vlahakes, G., Benjamin, E.J., Lunetta, K.L., Lubitz, S.A., Mirny, L., Milan, D.J., Ellinor, P.T.
- ID
- ZDB-PUB-171005-8
- Date
- 2017
- Source
- Circulation. Cardiovascular genetics 10(5): (Journal)
- Registered Authors
- Ellinor, Patrick, Milan, David J.
- Keywords
- atrial fibrillation, genetics, genome-wide association study, genomics, pulmonary veins
- MeSH Terms
-
- Action Potentials/genetics*
- Animals
- Animals, Genetically Modified
- Atrial Fibrillation*/genetics
- Atrial Fibrillation*/metabolism
- Atrial Fibrillation*/physiopathology
- Cell Line
- Homeodomain Proteins*/biosynthesis
- Homeodomain Proteins*/genetics
- Human Embryonic Stem Cells/metabolism*
- Human Embryonic Stem Cells/pathology
- Humans
- Mice
- Myocytes, Cardiac/metabolism*
- Myocytes, Cardiac/pathology
- Polymorphism, Single Nucleotide*
- Zebrafish
- PubMed
- 28974514 Full text @ Circ Cardiovasc Genet
Citation
Tucker, N.R., Dolmatova, E.V., Lin, H., Cooper, R.R., Ye, J., Hucker, W.J., Jameson, H.S., Parsons, V.A., Weng, L.C., Mills, R.W., Sinner, M.F., Imakaev, M., Leyton-Mange, J., Vlahakes, G., Benjamin, E.J., Lunetta, K.L., Lubitz, S.A., Mirny, L., Milan, D.J., Ellinor, P.T. (2017) Diminished PRRX1 Expression Is Associated With Increased Risk of Atrial Fibrillation and Shortening of the Cardiac Action Potential.. Circulation. Cardiovascular genetics. 10(5).
Abstract
Background Atrial fibrillation (AF) affects over 33 million individuals worldwide. Genome-wide association studies have identified at least 30 AF loci, but the mechanisms through which individual variants lead to altered disease risk have remained unclear for the majority of these loci. At the 1q24 locus, we hypothesized that the transcription factor PRRX1 could be a strong candidate gene as it is expressed in the pulmonary veins, a source of AF in many individuals. We sought to identify the molecular mechanism, whereby variation at 1q24 may lead to AF susceptibility.
Methods and results We sequenced a ≈158 kb region encompassing PRRX1 in 962 individuals with and without AF. We identified a broad region of association with AF at the 1q24 locus. Using in silico prediction and functional validation, we identified an enhancer that interacts with the promoter of PRRX1 in cells of cardiac lineage. Within this enhancer, we identified a single-nucleotide polymorphism, rs577676, which alters enhancer activity in a mouse atrial cell line and in embryonic zebrafish and differentially regulates PRRX1 expression in human left atria. We found that suppression of PRRX1 in human embryonic stem cell-derived cardiomyocytes and embryonic zebrafish resulted in shortening of the atrial action potential duration, a hallmark of AF.
Conclusions We have identified a functional genetic variant that alters PRRX1 expression, ultimately resulting in electrophysiological alterations in atrial myocytes that may promote AF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping