PUBLICATION
Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification
- Authors
- Hozumi, S., Aoki, S., Kikuchi, Y.
- ID
- ZDB-PUB-170928-16
- Date
- 2017
- Source
- Development (Cambridge, England) 144(21): 4015-4025 (Journal)
- Registered Authors
- Keywords
- Endoderm specification, JNK, MTOC, Nodal, Nuclear movement, Smad
- MeSH Terms
-
- Animals
- Body Patterning*
- Cell Movement
- Cell Nucleus/metabolism*
- Egg Yolk/metabolism
- Embryo, Nonmammalian/metabolism
- Endoderm/cytology
- Endoderm/embryology*
- Endoderm/metabolism
- Giant Cells/metabolism
- MAP Kinase Signaling System*
- Microtubule-Organizing Center/metabolism
- Microtubules/metabolism
- Models, Biological
- Nodal Protein/metabolism*
- Protein Transport
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Smad2 Protein/metabolism*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- PubMed
- 28947537 Full text @ Development
Citation
Hozumi, S., Aoki, S., Kikuchi, Y. (2017) Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification. Development (Cambridge, England). 144(21):4015-4025.
Abstract
Although asymmetric nuclear positioning is observed during animal development, the regulation and significance of this nuclear positioning in cell differentiation remains poorly understood. Using zebrafish blastulae, we provide evidence that nuclear movement toward the yolk syncytial layer, which comprises extraembryonic tissue, occurs in the first endoderm specified cells during endoderm specification. Nodal signaling is essential for nuclear movement, whereas nuclear envelope proteins are involved in the movement through the microtubule formation. The positioning of the microtubule organizing center, which is proposed to be critical for nuclear movement, is regulated by Nodal signaling and nuclear envelope proteins. The non-Smad JNK signaling pathway, which is downstream of Nodal signaling, regulates nuclear movement independent of the Smad pathway, and this nuclear movement is associated with Smad signal transduction toward the nucleus. Our study provides insights into the function of nuclear movement in Smad signaling toward the nucleus, and could be applied to the control of Transforming Growth Factor-β signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping