PUBLICATION
Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection
- Authors
- Vincent, W.J.B., Harvie, E.A., Sauer, J.D., Huttenlocher, A.
- ID
- ZDB-PUB-170921-13
- Date
- 2017
- Source
- PLoS One 12: e0179574 (Journal)
- Registered Authors
- Huttenlocher, Anna
- Keywords
- Macrophages, Larvae, Neutrophils, Streptococcal infections, Zebrafish, Immune cells, Immune response, Morpholino
- MeSH Terms
-
- Animals
- Epoxide Hydrolases/genetics
- Epoxide Hydrolases/metabolism
- Gene Deletion
- Immunity, Innate
- Inflammation/metabolism
- Leukotriene B4/metabolism*
- Macrophages/metabolism*
- NF-kappa B/metabolism
- Neutrophils/metabolism*
- Streptococcal Infections/metabolism*
- Streptococcus iniae
- Zebrafish
- PubMed
- 28658259 Full text @ PLoS One
Citation
Vincent, W.J.B., Harvie, E.A., Sauer, J.D., Huttenlocher, A. (2017) Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection. PLoS One. 12:e0179574.
Abstract
Immune cells sense and react to a multitude of factors including both host and microbe-derived signals. Understanding how cells translate these cues into particular cellular behaviors is a complex yet critical area of study. We have previously shown that both neutrophils and macrophages are important for controlling the fish pathogen Streptococcus iniae. Here, we report both host and bacterial determinants leading to the formation of organized macrophage aggregates as part of the host inflammatory response in a subset of infected larvae. Streptococcal capsule was a required signal for aggregate formation. Macrophage aggregation coincided with NFκB activity, and the formation of these aggregates is mediated by leukotriene B4 (LTB4) produced by neutrophils. Depletion, inhibition, or genetic deletion of leukotriene A4 hydrolase (Lta4h), which catalyzes the last step in LTB4 synthesis, resulted in the absence of macrophage aggregation. Larvae with impaired neutrophil function also had impaired macrophage aggregation; however, aggregate formation was partially rescued with the addition of exogenous LTB4. Neutrophil-specific expression of lta4h was sufficient to rescue macrophage aggregation in Lta4h-deficient larvae and increased host survival following infection. In summary, our findings highlight a novel innate immune response to infection in which specific bacterial products drive neutrophils that modulate macrophage behavior through eicosanoid signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping