PUBLICATION
Distribution and chronotropic effects of serotonin in the zebrafish heart
- Authors
- Stoyek, M.R., Jonz, M.G., Smith, F.M., Croll, R.P.
- ID
- ZDB-PUB-170802-20
- Date
- 2017
- Source
- Autonomic neuroscience : basic & clinical 206: 43-50 (Journal)
- Registered Authors
- Croll, Roger P., Jonz, Michael G.
- Keywords
- 5-Hydroxytryptamine, Chronotropy, Heart, Intracardiac nervous system, Serotonin, Zebrafish
- MeSH Terms
-
- Animals
- Cardiovascular Agents/pharmacology
- Electrocardiography
- Heart/drug effects
- Heart Rate/drug effects
- Heart Rate/physiology
- Immunohistochemistry
- Microscopy, Confocal
- Myocardium/cytology*
- Myocardium/metabolism*
- Neuroglia/cytology
- Neuroglia/drug effects
- Neuroglia/metabolism
- Neurons/cytology
- Neurons/drug effects
- Neurons/metabolism
- Serotonin/metabolism*
- Serotonin Agents/pharmacology
- Tissue Culture Techniques
- Zebrafish/anatomy & histology*
- Zebrafish/metabolism*
- PubMed
- 28757278 Full text @ Auton. Neurosci.
Citation
Stoyek, M.R., Jonz, M.G., Smith, F.M., Croll, R.P. (2017) Distribution and chronotropic effects of serotonin in the zebrafish heart. Autonomic neuroscience : basic & clinical. 206:43-50.
Abstract
Several lines of evidence suggest that serotonin (5-HT) has a regulatory role in cardiovascular function from embryogenesis through adulthood. However, the reported actions of 5-HT are often contradictory and include bradycardia or tachycardia, hypotension or hypertension, and vasodilation or vasoconstriction. Clarifying such cardiac effects requires further research and may benefit from utilizing a model simpler than the mammalian hearts traditionally used in these studies. In the present study, we describe the cardiac distribution and chronotropic responses of 5-HT in the zebrafish heart. A combined anatomical, electrophysiological, and pharmacological approach was used to investigate the involvement of 5-HT pathways, and to compare neural and direct myocardial pathways of biological action. Immunohistochemical methods revealed 5-HT in endocardial cells, glial-like cells, and intracardiac neurons in the atrium. Electrocardiogram (ECG) recordings combined with the administration of pharmacological agents demonstrated that 5-HT acted predominantly through direct myocardial pathways resulting in a reduction of heart rate. Overall, the results of this study contribute significant advances in the establishment of the zebrafish as a new model for studies of the role of 5-HT in autonomic cardiac control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping