PUBLICATION
TNFa/TNFR2 signaling is required for glial ensheathment at the dorsal root entry zone
- Authors
- Smith, C.J., Wheeler, M.A., Marjoram, L., Bagnat, M., Deppmann, C.D., Kucenas, S.
- ID
- ZDB-PUB-170518-9
- Date
- 2017
- Source
- PLoS Genetics 13: e1006712 (Journal)
- Registered Authors
- Bagnat, Michel, Kucenas, Sarah, Smith, Cody
- Keywords
- Pioneer axons, Spinal cord, Embryos, Neurons, Axons, Larvae, Zebrafish, Nerves
- MeSH Terms
-
- Animals
- Astrocytes/metabolism
- Axons/metabolism
- Central Nervous System/growth & development
- Central Nervous System/metabolism
- Ganglia, Spinal
- Gene Expression Regulation, Developmental
- Mice
- Neuroglia/cytology
- Neuroglia/metabolism*
- Neurons, Afferent/metabolism
- Peripheral Nervous System/growth & development
- Peripheral Nervous System/metabolism
- Receptors, Tumor Necrosis Factor, Type II/biosynthesis
- Receptors, Tumor Necrosis Factor, Type II/genetics*
- Signal Transduction
- Spinal Cord/growth & development
- Spinal Cord/metabolism
- Spinal Nerve Roots/growth & development
- Spinal Nerve Roots/metabolism*
- Tumor Necrosis Factor-alpha/biosynthesis
- Tumor Necrosis Factor-alpha/genetics*
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 28379965 Full text @ PLoS Genet.
Citation
Smith, C.J., Wheeler, M.A., Marjoram, L., Bagnat, M., Deppmann, C.D., Kucenas, S. (2017) TNFa/TNFR2 signaling is required for glial ensheathment at the dorsal root entry zone. PLoS Genetics. 13:e1006712.
Abstract
Somatosensory information from the periphery is routed to the spinal cord through centrally-projecting sensory axons that cross into the central nervous system (CNS) via the dorsal root entry zone (DREZ). The glial cells that ensheath these axons ensure rapid propagation of this information. Despite the importance of this glial-axon arrangement, how this afferent nerve is assembled during development is unknown. Using in vivo, time-lapse imaging we show that as centrally-projecting pioneer axons from dorsal root ganglia (DRG) enter the spinal cord, they initiate expression of the cytokine TNFalpha. This induction coincides with ensheathment of these axons by associated glia via a TNF receptor 2 (TNFR2)-mediated process. This work identifies a signaling cascade that mediates peripheral glial-axon interactions and it functions to ensure that DRG afferent projections are ensheathed after pioneer axons complete their navigation, which promotes efficient somatosensory neural function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping